
一文读懂差示扫描荧光法(DSF) - 知乎专栏
差示扫描荧光法(Differential Scanning Fluorimetry, 简称DSF),是在荧光定量PCR仪上缓慢加热样品,在加热的过程中检测荧光染料与结构发生改变的蛋白质相结合的量,来评价蛋白质热稳定性的方法。
nanoDSF 微量差示扫描荧光法表征蛋白配体互作 - 知乎
2022年9月20日 · 差示扫描荧光法(DSF),也被称为thermal shift assay(TSA),是表征蛋白热稳定性的常用方法之一,广泛应用于蛋白配体互作表征,突变体、缓冲液、去垢剂筛选等领域。
Protocol for performing and optimizing differential scanning ...
2023年12月15日 · Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for performing and optimizing DSF experiments. We describe steps for designing and performing the experiment, analyzing data, and optimization.
Theory and applications of differential scanning fluorimetry in …
2020年1月31日 · We illustrate the uses of DSF in protein buffer optimization for stability, refolding, and crystallization purposes and provide several examples of each. We also show the use of DSF in a more downstream application, where it is used as an in vivo validation tool of ligand-target interaction in cell assays.
Protein-adaptive differential scanning fluorimetry using ... - Nature
2024年5月14日 · Differential scanning fluorimetry (DSF) is a technique that reports protein thermal stability via the selective recognition of unfolded states by fluorogenic...
Differential Scanning Fluorimetry (DSF) | Center for …
Conventional Differential Scanning Fluorimetry (DSF) uses a real-time PCR instrument to monitor thermally induced protein denaturation by measuring changes in fluorescence of a dye that binds preferentially to unfolded protein (such as Sypro Orange, which binds to hydrophobic regions of proteins exposed by unfolding).
A beginner’s guide to differential scanning fluorimetry
2025年1月29日 · Differential scanning fluorimetry (DSF) is a technique used to determine a protein’s stability. In a typical DSF experiment, a purified protein is heated in the presence of a fluorescent dye and the resulting temperature vs fluorescence plot is used to calculate its apparent melting temperature.
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全新一代差示扫描荧光仪SUPR-DSF
使用SUPR-DSF 获得NISTmAb (NIST 单抗标准品) 的差示扫描荧光数据,并将结果与差示扫描量热法 (DSC) 所获得的数据进行比较。 SUPR-DSF 可以解析NISTmAb所有的三个结构域: CH2(69°C),CH3 (83°C) ,和Fab区域(94°C)。 SUPR-DSF 的最高扫描温度为105°C,可以精确测量异常稳定的Fab区域熔解温度,而其他DSF平台的扫描最高温度一般仅为95°C,容易丢失单抗Fab区域去折叠变性的重要信息 ( 图5(a) )。 15 个单点突变体。 在1.5小时内,384 孔的数据)。 用模型对数.
重组羧肽酶B(RCPB)说明书 - imed-bio.com
CPB的最适pH为8.0,偏酸或偏碱均会造成活性下降,pH接近5.0时活性接近零,pH>12.0时活性完全丧失。 CPB在一定温度内( 重组羧肽酶B(RCPB)说明书
Int J Surg:武汉协和医院麻醉姚尚龙教授与心外董念国教授团队合 …
2023年10月8日 · 首次开发了一个基于蛋白预警因素和临床预警因素的CPB-ARDS预警模型,经外部队列验证该模型预测性能良好:分别建立模型训练队列和验证队列,筛选临床预警因素,联合ELISA验证后的预警蛋白建立CPB-ARDS的列线图预警模型,应用于CPB-ARDS的个体化预测;开 …