差示扫描荧光法（Differential Scanning Fluorimetry, 简称DSF），是在荧光定量PCR仪上缓慢加热样品，在加热的过程中检测荧光染料与结构发生改变的蛋白质相结合的量，来评价蛋白质热稳 …

Differential Scanning Fluorimetry (DSF) measures protein unfolding by monitory changes in fluorescence as a function of temperature. Conventional DSF uses a hydrophobic fluorescent …

The most common method for measuring protein thermal shifts is differential scanning fluorimetry (DSF). DSF methodology includes techniques such as nanoDSF, [1][2] which relies on the …

2023年12月15日 · Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a …

2022年9月20日 · 差示扫描荧光法（DSF），也被称为thermal shift assay（TSA），是表征蛋白热稳定性的常用方法之一，广泛应用于蛋白配体互作表征，突变体、缓冲液、去垢剂筛选等领域。

2024年5月14日 · Differential scanning fluorimetry (DSF) is a technique that reports protein thermal stability via the selective recognition of unfolded states by...

2020年1月31日 · DSF is a cost-effective, parallelizable, practical, and accessible biophysical technique widely used as a method to track both protein folding state and thermal stability. It …

2022年3月4日 · 差示扫描荧光法 (differential scanning fluorimetry, DSF) 是一种方便快捷的高通量药物筛选及靶标发现的方法，该方法具有蛋白样品损耗少、通量高、温度变化范围广及数据准 …

Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for performing …

2020年12月4日 · Differential scanning fluorimetry (DSF) can be used to detect the binding of a small molecule ligand to a purified target protein. Upon binding with certain ligands, the protein …