Ubiquitin-protein ligase E3A (UBE3A) also known as E6AP ubiquitin-protein ligase (E6AP) is an enzyme that in humans is encoded by the UBE3A gene. This enzyme is involved in targeting proteins for degradation within cells.

E6-AP 是一种 HECT 结构域 E3 泛素连接酶，会与丙型肝炎病毒 (HCV) 核心蛋白相互作用，并靶向这种蛋白进行降解。 HCV 核心蛋白对包装病毒 DNA 和其他细胞进程非常重要。 E6-AP 还会与人乳头瘤病毒 16 和 18 型的 E6 蛋白相互作用，并靶向 p53 抑癌基因蛋白进行降解。 HACE1 是一种 E3 泛素连接酶，也是一种抑癌基因。 HACE1 的异常甲基化频繁在维尔姆斯瘤和结直肠癌细胞中被发现。 HECTD1 是神经管闭合和间质正常发育所必需的 E3 泛素连接酶。 HECTD2 是一 …

The ubiquitin ligase E6-AP (E6-associated protein) represents a prime example for the notion that deregulated modification of proteins with ubiquitin contributes to the development of human disease: loss of E6-AP function by mutation is responsible for the development of AS (Angelman syndrome), a neurological disorder, and unscheduled ...

2024年4月26日 · Here we show that E6AP and the E6AP/E6 complex exist, respectively, as a monomer and a dimer of the E6AP/E6 protomer. The short α1-helix of E6AP transforms into a longer helical...

E6-AP is a hect (homologous to E6-associated protein carboxy-terminal domain) domain containing E3 ubiquitin ligase that possesses two independent separable functions; a coactivation function and an ubiquitin-protein ligase activity. Being a component of the ubiquitin-proteasome pathway, it is postulated that E6-AP may orchestrate the dynamics ...

2018年10月25日 · Deregulation of the ubiquitin ligase E6AP is causally linked to the development of human disease, including cervical cancer. In complex with the E6 oncoprotein of human papillomaviruses, E6AP...

2020年3月10日 · Regulated proteolysis by proteasomes involves ~800 enzymes for substrate modification with ubiquitin, including ~600 E3 ligases. We report here that E6AP/UBE3A is distinguished from other E3...

2024年5月15日 · 该研究利用冷冻电镜技术分别解析了E6AP和E6结合E6AP不同构象复合物结构，并结合分子动力学模拟分析和生化实验，系统揭示了E6AP活性动态调控的分子机制。 E6AP是HECT型泛素连接酶家族的创始成员，最初因与人乳头瘤病毒（human papillomavirus, HPV）的癌蛋白E6相互作用而被发现。 来自高危型HPV的E6会劫持E6AP的泛素连接酶活性，导致肿瘤抑制因子p53的异常泛素化与降解，其结果与多种HPV阳性癌症的发生发展有关，包括超过90%的宫 …

2013年10月5日 · In this review, we delineate genetic and biochemical knowledge of E6AP and we focus on its role in the pathobiology of neuro-developmental and neuro-aging diseases; bringing to light important gaps of knowledge related to the involvement of its well-studied ligase function versus the much less studied nuclear receptor transcriptional coactivatio...

The E6-associated protein (E6-AP), although originally identified as a ubiquitin ligase, has recently been shown to function as a coactivator of steroid receptor-dependent gene expression in in vitro assays. In order to determine whether E6-AP acts as a …