In general, Br-C, E74, E75, and E93 positively affect 20E signaling (8). As a potent HTH transcription factor, E93 binds several 20E response genes and PCD genes on polytene chromosomes in Drosophila (16). The expression of these genes is reduced in E93 mutants, whereas E93 overexpression up-regulates some of the genes (13, 16).

2021年2月1日 · The ecdysone-inducible early gene E74 (Eip74EF) encodes an ETS (erythroblast transformation specific) transcription factor that mediates the 20E response in gene regulation. In mosquitoes, there are two isoforms, E74A and E74B, that act by differentially activating or repressing 20E-responsive genes ( 22 , 23 ).

2017年12月19日 · 昆虫的变态发育包括幼虫蜕皮和幼虫-蛹-成虫变态，均为蜕皮激素（20-hydroxyecdysone，20E）诱导产生；而倍半萜烯类激素-保幼激素（Juvenile hormone，JH）在幼虫蜕皮期拮抗20E作用而维持幼虫性状，从而阻止变态提前发生。

2002年4月25日 · We report characterization of two isoforms of the mosquito early E74 gene (AaE74), which have a common C-terminal Ets DNA-binding domain and unique N-termini. They exhibited a high level of identity to Drosophila E74 isoforms A and B and showed structural features typical for Ets transcription factors.

While indirect repression involves E74, EcR binds to an ecdysone response element different from those utilized in 20E-mediated gene activation to exert direct repressive action. Moreover, liganded EcR recruits a corepressor Mi2, initiating chromatin compaction.

2004年4月15日 · Vitellogenesis is regulated by the 20-hydroxyecdysone (20E) regulatory hierarchy, in which the Ets-domain protein E74 is a key transcriptional regulator. The mosquito AaE74 gene encodes two isoforms—AaE74A and AaE74B. Both AaE74 isoforms are 20E-inducible early gene products.

2022年10月1日 · HcE74 knockdown reduced chitin content in midgut and epidermis. E74 is a key transcription factor induced by 20E, which plays a broad role in many physiological events during insect growth and development, including vitellogenesis, organ remodeling and new tissue formation, programmed cell death and metamorphosis.

Five Atg genes were potentially 20E primary-responsive, and a 20E response element was identified in the Atg1 (ortholog of human ULK1) promoter region. Furthermore, RNAi knockdown of 4 key genes (namely Br-C, E74, HR3 and βftz-F1) in the 20E-triggered transcriptional cascade reduced autophagy and downregulated Atg genes to different levels.

2015年11月6日 · Importantly, the two HTH domains in E93 are critical for inducing the expression of a subset of 20E response genes, including EcR, USP, E74, Br-C, and Atg1. By contrast, the LLQHLL and PLDLSAK motifs in E93 inhibit its transcriptional activity.

2023年6月26日 · We found that ECR gene expression peaked in the 7-day-old larvae, then decreased gradually from the pupae stage. 20E slowly reduced food consumption and then induced starvation, resulting in small-sized adults. In addition, 20E induced ECR expression to regulate larval development time.