EGF-responsive interleukin-6 and interleukin-8 were synergistically enhanced by EGF stimulation and mechanical strain. We show here in immortalized and primary BMSC that EGFR signaling enhances mechanotransduction, indicating that the EGF system is a mechanosensitizer in BMSC.

HB-EGF promoted BMSC proliferation via the Akt1 and Erk pathways but inhibited BMSC differentiation via restraining Smad1/5/8 activation. However, Dermo1-HB-EGF mice showed normal osteoclastogenesis and bone resorption.

2017年10月1日 · We investigated the effects of EGFR signaling in telomerase-immortalized BMSC, transfected with a luciferase reporter, comprising a mechanoresponsive AP1 element, using ligands, neutralizing antibodies and EGFR inhibitors on mechanotransduction and we found that EGF via EGFR increased the response to mechanical strain.

Our findings demonstrate that cyclic strain and electrical co-stimulation have a synergetic effect on EGF and FGF2-induced rat BMSC neural differentiation by upregulating neural markers and neurotropic mediators and increase calcium influx, intracellular cAMP, and phosphorylation of ERK1/2 and AKT.

HB‐EGF inhibits BMSC in vitro osteogenic and chondrogenic differentiation via Smad1 signaling. To understand the molecular mechanisms by which HB‐EGF regulates BMSC proliferation and differentiation, we compared the activation of HB‐EGF downstream signaling pathways in BMSCs isolated from Dermo1‐HB‐EGF and

2018年12月14日 · HB-EGF通过Akt1和Erk途径促进BMSC增殖，但通过抑制Smad1 / 5/8激活抑制BMSC分化。 但是，Dermo1-HB-EGF小鼠表现出正常的破骨细胞生成和骨吸收。 这些结果揭示了自分泌或旁分泌HB-EGF在间充质基质细胞增殖和分化中的重要功能，并建议需要严格控制EGF信 …

In this concept paper, we focus on the potential use of epidermal growth factor (EGF), prototypal growth factor for enhancing the harvesting and/or differentiation of MSCs. Soluble EGF was shown to augment MSC proliferation while preserving early progenitors within MSC population, and thus did not induce differentiation.

2021年12月12日 · BMSCs supported myeloma colony formation by secreted epidermal growth factor (EGF), which bound with its receptor (EGFR) on myeloma cells and activated Mek/Erk cell signaling, PI3K/Akt cell signaling, and hedgehog pathway. ALCAM could also bind with EGFR, block EGF from binding to EGFR, and abolish EGFR-initiated cell signaling.

2015年11月26日 · 摘要： 目的 探究胰岛素样生长因子结合蛋白4（IGFBP4）对骨髓间充质干细胞（BMSC）增殖和神经分化的影响。 方法 通过全骨髓贴壁法分离培养BMSCs，免疫荧光检测IGFBP4在P1、P4、P6、P8和P10 BMSCs的表达。 取第4代BMSC细胞，分为单纯培养基组和添加IGFBP4组，用CCK8法检测吸光光度值。 再将BMSC细胞分为3组，诱导培养基NeuroCult培养再分别添加EGF+bFGF和EGF+bFGF+IGFBP4，诱导其向神经祖细胞分化，免疫荧光染色检 …

2013年10月1日 · The objective of this work was to study the effect of epidermal growth factor (EGF) induced secretions of angiogenesis factors in adipose-derived stem cells (ADSCs) and the involvement of mitogen-activated protein kinases (MAPK).