EPU is a Thermo Fisher Scientific software product for automated high quality data acquisition in a Single Particle Analysis (SPA) workflow. SPA is an approach to 3D image creation, during …
Cryo EM Software | Smart EPU Software | Cryo EM Acquisition …
Thermo Scientific Smart EPU Software is an innovative, easy-to-use data acquisition solution for single particle analysis (SPA) that focuses on automation and user guidance to increase …
Click Preview and watch EPU perform a few auto functions and collect the images as defined in the template. If something is amiss, you’ll have an opportunity to make adjustments before …
在Reference Image Manager 点Measure dose ,确认剂量4-10e/px/s 绿色内,Exposure time可以选择收数据的曝光时间或者默认的10都可以; 去背底结束后再次检查背底是否良好,如果良好则可 …
Collecting data on cryo-EM grids on a Falcon 4 detector with EPU software (ThermoFisher Scientific) 2.1. EPU is an automated data collection software from ThermoFisher Scientific …
Data Collection with EPU Standard Operating Procedure (K3) version 1.1 Auth Purpose K3 Definitions: utom ted data collection software from ThermoFishe Sci 2.2. TEMUI is the user …
Collection, pre-processing and on-the-fly analysis of data for high ...
2018年11月28日 · This protocol describes a pipeline for data collection, pre-processing and on-the-fly analysis for single-particle cryo-electron microscopy using EPU software and two direct …
What’s new on Smart EPU update: Image Shift Calibration
2023年9月20日 · This tutorial demonstrates the latest features of Smart EPU Software for SPA included: TIFF LZW output for Falcon-4i cameras, guided Image Shift Calibration,...
2018年7月11日 · Main steps for quick reference: Make sure microscope is properly aligned and energy filter is tuned Open EPU, center all beams, double check settings Do image shift …
EPU-CM-Blog-Discovery-Viewer_1024x725 - Life in Atomic …
Figure 2. Discovery Viewer, a navigation tool specific for cryo-EM, is an add-on that helps to precisely determine where specific images were captured on a sample during the single …