2018年4月27日 · In the present study, we identified a novel PGC-specific marker gene, C2EIP 13, 14, and characterized its molecular function in detail. We previously reported that C2EIP was not noticeably...

In the present study, we identi fied a novel PGC-speci fic marker gene, C2EIP13,14, and characterized its molecular function in detail. We previously reported that C2EIP was not noticeably...

2018年6月5日 · The ESC marker OCT4, PGC marker CVH, and SSC marker integrin α6 were DAPI staining and immunofluorescence (IF) staining. (C) DNA methylation trend through different development stages of chick germ cells.

2016年2月26日 · 第一步，将小鼠胚胎干细胞（esc）诱导分化成原始生殖细胞样细胞（pgclc）；第二步，研究人员模拟原始生殖细胞（pgc）体内发育的“自然组织环境”，即与睾丸细胞共培养并添加性激素如睾酮等。

2015年3月21日 · Through our study, we found that in the differentiation process of ESC to PGC and PGC to SSC, the differentially expressed genes were enriched on 16 and 15 amino acid metabolic pathways, respectively.

2015年4月1日 · Cell sorting was used to obtain highly purified embryonic stem cells (ESCs), primitive germ cells (PGCs), and spermatogonial stem cells (SSCs). The total RNA was then extracted from each cell type. The transcriptions of ESCs, PGCs, and SSCs were sequenced by DNA microarray and mRNA sequencing (RNA-seq).

2018年4月27日 · Our data suggest that C2EIP is a novel, specific indicator of PGC generation whose gene product regulates embryonic stem cell differentiation by activating the HH signaling pathway via PTCH2...

本研究旨在探讨在鸡胚干细胞（ESCs）分化为精原干细胞（SSCs）过程中细胞色素P450对异源生物代谢的调控机制，并完善鸡胚干细胞（cESCs）诱导分化为SSCs的系统。 体外。 我们在高度纯化的雄性ESC，雄性原始生殖细胞（PGC）和与雄性生殖细胞分化相关的SSC中进行了RNA-Seq。 其中，选择了细胞色素P450异种生物的代谢，并用Venny分析了男性ESC与男性PGC，男性PGC与SSC，男性ESC与SSC之间的异种生物，并挖掘了几个候选差异表达基因（DEG）。 …

Download scientific diagram | C2EIP encodes a cytoplasmic protein that is differentially expressed in PGC. a Transcriptome analysis of ESC, PGC, and SSC. The differential expression of genes in ...

2019年11月1日 · This study demonstrated different effects of bone morphogenetic protein 4 (BMP4) and retinoic acid (RA) signaling on the induction of germ cell formation in chickens. In vitro, BMP4 significantly promoted primordial germ cell (PGC) formation, while RA promoted spermatogonial stem cell (SSC) formatio …