oriP is a 1.7-kb region of the Epstein-Barr virus (EBV) chromosome that supports the replication and stable maintenance of plasmids in human cells. oriP contains two essential components, called the DS and the FR, both of which contain multiple binding sites for the EBV-encoded protein, EBNA-1.

2002年10月14日 · Use of oriP/EBNA1-based episomal vectors can potentially revolutionize basic chromosome research ranging from function and gene expression to transgene production of therapeutics...

All of these functions involve EBNA1 binding to specific DNA recognition sites in the EBV latent origin of DNA replication (oriP). In addition, EBNA1 has been shown to alter the cellular environment in multiple ways that might facilitate viral infection and contribute to cell immortalization and survival.

在本研究中，我们发现携带oriP的质粒需要EBNA-1才能有效复制，即使将质粒引入细胞系143B和293后仅进行了2天的检测。 重要的是，使用293细胞证明，质粒的保留功能EBNA-1和FR对复制质粒的积累没有显着贡献，并且DS在缺少FR的情况下支持有效的EBNA-1依赖性复制。

2021年2月4日 · EBNA1 tyrosine 518 (Y518) is essential for crosslinking to oriP and functionally required for episome maintenance and generation of EBV-transformed lymphoblastoid cell lines (LCLs). Mechanistically, Y518 is required for replication fork termination at oriP in vivo and for formation of SDS-resistant complexes in vitro.

oriP is a 1.7-kb region of the Epstein-Barr virus (EBV) chromosome that supports the replication and stable maintenance of plasmids in human cells. oriP contains two essential components, called the DS and the FR, both of which contain multiple ...

2001年2月6日 · EBV-encoded nuclear antigen-1 (EBNA-1) binding to a cis-acting viral DNA element, oriP, enables plasmids to persist in dividing human cells as multicopy episomes that attach to chromosomes during m...

During Epstein-Barr virus latent infection of B lymphocytes in vitro, six viral nuclear antigens (EBNAs) are expressed from one of two promoters, Cp or Wp, whose activities are mutually exclusive. Upon infection, Wp is initially active, followed by a …

The EBNA1 protein of Epstein-Barr virus enables plasmids carrying oriP both to duplicate and to segregate efficiently in proliferating cells. EBNA1 recruits the origin recognition complex (ORC) to establish a replication origin at one element of oriP, DS (dyad symmetry); at …

EBV-encoded nuclear antigen-1 (EBNA-1) binding to a cis-acting viral DNA element, oriP, enables plasmids to persist in dividing human cells as multicopy episomes that attach to chromosomes during mitosis.