20874-1-AP targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

E-Cadherin是一种单通道I型跨膜糖蛋白，定位于上皮细胞的黏附连接 (AJ)和基底外侧膜，由一个较大的胞外结构域、一个跨膜段和一个保守的胞质结构域组成。 定位于细胞质膜外表面的一种α-分泌酶可裂解E-Cadherin，E-Cadherin并被包括基质金属蛋白酶 (MMP-3、MMP-7、MMP-9、MT1-MMP)、解整合素、金属蛋白酶 (ADAM10、ADAM15)、纤溶酶和激肽释放酶7在内的几种蛋白酶催化，将成熟的120kDa左右的E-Cadherin蛋白转化为细胞外的N-端80kDa左右的可溶性E …

E-Cadherin (24E10) Rabbit mAb detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST.

2021年11月28日 · 本文作者将从“E-cadherin和N-cadherin在正常细胞、组织及肿瘤样本中的表达情况”以及“分别分析E-cadherin、N-cadherin 在 EMT 及肿瘤侵袭、转移中的作用”两个角度给出答案。最后，对于WB实验中两个蛋白的检测，也指出了操作的关键点。

了解E-Cadherin的WB解决方案，掌握常见WB指标试验的Tips，提高实验的准确性。

Polyclonal Antibody for studying E-Cadherin. Validated for IF, IHC, IP, WB. Highly specific and rigorously validated in-house, E-Cadherin Antibody (CST #4065) is ready to ship.