2023年10月25日 · Results: EFR3A was identified as a candidate protein that interacts with flotillin-2. Moreover, this newly discovered interaction was demonstrated via overlay assay using recombinant EFR3A and flotillin-2.

2024年12月24日 · EFR3A (EFR3 Homolog A) is a Protein Coding gene. Diseases associated with EFR3A include Developmental Malformations-Deafness-Dystonia Syndrome and Autism Spectrum Disorder. Gene Ontology (GO) annotations related to this gene include binding. An important paralog of this gene is EFR3B.

A Effect of EFR3A gene silencing on activation of EGF-induced EGF receptor and phospholipase PLCγ1 in HeLa cells via western blotting analysis. B Effect of MβCD on EGFR and PLCγ1 phosphorylation upon EGF treatment of WT HeLa cells. Fifteen micrograms of cell lysates was loaded on the 10% SDS-PAGE gel.

2023年10月25日 · EFR3A was identified as a candidate protein that interacts with flotillin-2. Moreover, this newly discovered interaction was demonstrated via overlay assay using recombinant EFR3A and flotillin-2. EFR3A is a stable component of the detergent-resistant membrane fraction of HeLa cells, and its presence was sensitive to the removal of cholesterol.

The PLCG1 activating variant resulted in enhanced NF-κB and type II interferon pathways in T cells, and hyperactivated NF-κB and type I interferon pathways in monocytes. Treatment with either PLCγ1 inhibitor or Janus kinase inhibitor reversed the upregulated gene expression profile in …

2021年9月9日 · Disrupting EFR3A or PI4KA reduces phosphatidylinositol-4-phosphate, phosphatidylserine, and KRAS levels at the plasma membrane, as well as oncogenic signaling and tumorigenesis, phenotypes...

2024年12月20日 · The EFR3A C terminus undergoes a disorder-order transition upon binding to the PI4KA complex, with an unexpected direct interaction with both TTC7B and FAM126A. Complex disrupting mutations in TTC7B, FAM126A, and EFR3 decrease PI4KA recruitment to the plasma membrane.

EFR3A being a putative otillin-2 partner was conrmed via western blotting (WB) with anti-EFR3 antibodies, co-immunoprecipitation (Co-IP) on HeLa cells, and overlay assay on puried recombinant proteins. To explore the possibility of the role of this interac-tion in raft domain organization, silencing of EFR3A gene expression in a HeLa cell line

2015年1月1日 · Here, we find that Efr3s plays a role in maintaining responsiveness to the type-I angiotensin II (AngII) receptors. siRNA-mediated depletion of EFR3A and EFR3B impaired the sustained phase of cytosolic Ca 2+ response to high concentration of AngII in HEK293 cells that express wild type but not truncated AGTR1 (AT1a receptor), missing the ...

2022年11月20日 · Results: Thereby EFR3A was identified as a candidate protein that interacts with flotillin-2. Moreover, this newly discovered interaction was demonstrated via overlay assay using recombinant...