2010年11月19日 · Erk activation enhanced the association of Runx2 with p300 and Smad1. Taken together these results indicate that Erk signaling increases Runx2 stability and transcriptional activity, partly via increasing p300 protein levels and histone acetyltransferase activity and subsequently increasing Runx2 acetylation by p300.

2009年11月11日 · ERK-Runx2 complexes were detected in nuclear extracts from MC-4 cells that have high endogenous levels of both proteins (panel A) as well as in COS7 cells transfected with affinity-tagged Runx2 (panels B–D). To identify the Runx2 region responsible for ERK binding, COS7 cells were transfected with wild type HA-tagged Runx2, Runx2 with S301A ...

As suggested in the present study, phosphorylation of RUNX2 may affect its ability to bind P-ERK and p300/CBP in that chromatin binding of these factors was dramatically reduced in cells treated with a MAPK inhibitor or transfected with a phosphorylation-deficient RUNX2 mutant (RUNX2 SA).

2016年1月25日 · Here we demonstrate Runx2-mediated IGF-1Rβ-pAkt and AMPK-Erk1/2 (extracellular-regulated protein kinase) signaling crosstalk in survival of bone-derived breast cancer cells. Importantly, Runx2...

Runt-related transcription factor 2 (RUNX2) is a key transcription factor for bone formation and osteoblast differentiation. Various signaling pathways and mechanisms that regulate the expression and transcriptional activity of RUNX2 have been thoroughly investigated since the involvement of RUNX2 was first reported in bone formation.

2010年2月5日 · In this study, we found that FGF2 treatment increased the protein level of exogenously overexpressed Runx2 and that this increase is reversed by ERK inhibitors. In contrast, overexpression of constitutively active MEK strongly increased the Runx2 protein level, which paralleled an increase in Runx2 acetylation.

2009年11月20日 · These results emphasize the important role played by Runx2 phosphorylation in the control of osteoblast gene expression and provide a mechanism to explain how physiological signals acting on bone through the ERK/MAPK pathway can stimulate osteoblast-specific gene expression.

2019年4月16日 · 在用IL‐1β或纤连蛋白片段（Fn-fs）刺激后，我们发现ERK抑制降低了OA-SF中Runx2活化和ADAMTS-12表达，同样减少了Fn-fs导致的COMP降解。 通过DKK1阻断Wnt信号通路亦可减少OA-SF中ADAMTS‐7表达和COMP降解。 另外，Wnt7B的表达由IL‐1β和其自身诱导，还可增加ADAMTS‐7。 我们的研究结果有助于开发针对 ADAMTS‐7/12的疾病缓解OA药物，用于预防细胞外基质成分的降解，如COMP。

2009年11月20日 · In this study, we used a combination of in vitro and in vivo phosphorylation analysis, mass spectroscopy, and functional assays to identify two sites at Ser 301 and Ser 319 within the proline/serine/threonine domain of Runx2 that are required for this regulation. These sites are phosphorylated by activated ERK1 in vitro and in cell culture.

RUNX2, an essential transcription factor for osteoblast differentiation and bone formation is activated by ERK/MAP kinase-dependent phosphorylation. However, relationship between these early events and specific epigenetic modifications of chromatin during osteoblast differentiation have not been pre …