Protocol for the isolation and purification of human follicular ...
2023年9月15日 · In this protocol, we detail how to isolate and purify human follicular dendritic cells (FDCs) from lymphoid tissues. FDCs play a vital role in antibody development by …
流式细胞分选原理 - 知乎 - 知乎专栏
流式分选细胞，又可称之为 荧光激活细胞分选 （Fluorescence-activated cell sorting, FACS），一种利用荧光标记和 流式细胞仪 对细胞进行检测和分选的技术，简而言之：利用 荧光素 标记细 …
Isolation and Characterization of Mouse and Human Follicular …
2017年6月7日 · Follicular dendritic cells (FDCs) reside in the B cell follicles of secondary and tertiary lymphoid tissues where they play key roles in the development and maintenance of …
FACS（流式细胞荧光分选技术）_百度百科
仪器中一系列光学系统 (透镜、光阑、滤片和检测器等)收集荧光、光散射、光吸收或细胞电阻抗等信号，计算机系统进行收集、储存、显示并分析被测定的各种信号，对各种指标做出统计分析 …
荧光激活细胞分选 - 丁香园论坛 - DXY.cn
荧光激活细胞分选 (FACS),也叫做流式细胞分选 (FCMS, flow cytometry sorting)。 该技术是利用流式细胞分选仪,以高能量激活照射高速流动状态下被荧光色素染色的单细胞或微粒,测量其产生 …
Follicular Dendritic Cell Isolation and Loading of Immune Complexes
Follicular dendritic cells (FDCs) are stromal cells that are centrally located within B cell follicles of lymph nodes and other lymphoid organs such as the spleen. Due to their relative low …
Characterization of human FDCs reveals regulation of T cells and ...
2021年10月4日 · Stromal-derived follicular dendritic cells (FDCs) are essential for germinal centers (GCs), the site where B cells maturate their antibodies. FDCs present native antigen to …
Isolation and Characterization of Mouse and Human Follicular Dendritic ...
FDCs trap native antigens for extended periods of time in the form of immune complexes which critcally regulate germinal center reactions in health and disease. Here, we describe how to …
活细胞荧光激活细胞分选术 - Abcam中文官网
用于活细胞的荧光激活细胞分选术 (facs) 根据荧光标记将一个细胞群分为多个亚群。 在流式细胞仪中，这种分选的机制相比非分选分析更为复杂。 根据所染荧光团的类型，可将荧光团偶联抗 …
流式细胞术（FC）和流式细胞荧光分选技术（FACS）实验技巧
运行流式细胞术(fc)，尤其是流式荧光激活细胞分选 (facs)，对于新的研究人员来说可能比较棘手。 以下提示和技巧将可以帮助您的团队在进行 FC 和 FACS 时节省时间和精力，并提高整个实验 …