Plasmid pCAMBIA-FLS2prom::FLS2-3xmyc-GFP from Dr. Silke Robatzek's lab contains the insert FLS2 and is published in Genes Dev. 2006 Mar 1;20(5):537-42. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format

2015年1月27日 · Our results clearly demonstrate that local expression of FLS2 in roots leads to flg22-dependent downstream MAP kinase phosphorylation, the production of ROS and the induction of defense marker genes in isolated roots.

该文综述了FLS2 向细胞膜运输及在细胞膜超微区中的定位、FLS2的内吞,以及选择性自噬和泛素- 蛋白酶体系统对FLS2的降解等,并分析了该领域今后研究的主要内容和方向。 关键词:FLS2 ;免疫信号转导;内吞;选择性自噬;泛素-蛋白酶体系统中图分类号:Q942.5 ;S432.2. Abstract: Cell surface-localized pattern recognition receptors (PRRs) sense microbes through perception of pathogen-associated molecular patterns (PAMPs) to trigger the first layer of immunity (PTI).

2025年2月26日 · This study focuses on the question how and where information acquired by FLS2 perception of flg22 is transformed into electrical signals crucial for generation of local and systemic defense responses.

2006年3月1日 · A variant FLS2 mutated in Thr 867, a potential phosphorylation site, binds flg22 normally, but is impaired in flg22 responses and FLS2 endocytosis. We propose that plant cells regulate pathogen-associated molecular pattern (PAMP)-mediated PRR activities by subcellular compartmentalization.

2006年3月1日 · Here, we demonstrate that a functional fusion of FLS2 to the green fluorescent protein (GFP) resides in cell membranes of most tissues. Stimulation with the flagellin epitope flg22 induces its transfer into intracellular mobile vesicles, followed by degradation.

2024年3月11日 · The plant RLK FLS2 is an indispensable component of the immune signaling pathway that regulates plant responses to bacterial flagellin peptides. Although the structural mechanism by which AtFLS2 co-recognizes bacterial flagellin peptides with AtBAK1 has been clarified, the structural basis by which the intracellular domain of plant FLS2 ...

2018年12月5日 · Here, we show that flagellin induces the conjugation of the SMALL UBIQUITIN-LIKE MODIFIER (SUMO) protein to FLS2 to trigger release of BIK1. Disruption of FLS2 SUMOylation can abolish immune...

2020年3月2日 · EXO70B1-GFP plants showed increased FLS2 accumulation at the PM and therefore enhanced FLS2 signaling. The EXO70B1-mediated trafficking of FLS2 to the PM is partially independent of the PENETRATION 1 (PEN1)-containing secretory pathway.

2024年7月24日 · Importantly, we found that the phosphorylation of Ser-938 enhanced flg22-induced FLS2 internalization and immune responses, demonstrating that the phosphorylation may activate flg22-triggered immunity through partitioning FLS2 into functional At Rem1.3-associated nanodomains, which fills the gap between the FLS2 S938 phosphorylation and FLS2-med...