2022年9月8日 · 衍生自单异构体6-羧基荧光素的6-FAM CE Phosphoramidite、6-HEX CE Phosphoramidite和6-TET-CE Phosphoramidite均可用于寡核苷酸5’端的有效标记。 另外两种可用于寡核苷酸荧光素标记的亚磷酰胺单体：6-Fluorescein-CE Phosphoramidite和Fluorescein-CE Phosphoramidite，这两种单体都包含与6-FAM CE ...

衍生自单异构体6-羧基荧光素的6-FAM CE Phosphoramidite、6-HEX CE Phosphoramidite和6-TET-CE Phosphoramidite均可用于寡核苷酸5’端的有效标记。 另外两种可用于寡核苷酸荧光素标记的亚磷酰胺单体：6-Fluorescein-CE Phosphoramidite和Fluorescein-CE Phosphoramidite，这两种单体都包含与6-FAM CE Phosphoramidite相同的荧光染料，但链接骨架不同。 6-Fluorescein-CE Phosphoramidite具有1,3二醇结构，其中额外的OH受DMTr保护。

Labelling the 3'-end of an oligo with fluorescein can be achieved by a variety of solid supports, with spacer and dT options. Notably, the 3'-(6-FAM) CPG also allows the effective blockage of the 3'-terminus from polymerase extension, as well as exonuclease activity.

3′-(6-FAM)-CPG; Synonyms: 1-Dimethoxytrityloxy-3-(di-O-pivaloyl-fluorescein-6-carboxamidopropyl)-propyl-2-O-succinoyl-long chain alkylamino-CPG,6-Carboxyfluorescein bound to controlled pore glass; find Sigma-Aldrich-40546 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich

FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5' or 3' end of oligos.

Fluorescein CPG has traditionally been used to add the fluorescein label at the 3'-terminus. The analogous structure, 3'-(6-Fluorescein) CPG, prepared using 6-FAM, is now also available, along with 6-Fluorescein Serinol CPG. We also offer 3'-(6-FAM) CPG and Fluorescein-dT CPG, both derivatives of 6-carboxyfluorescein (6-FAM).

化学名称: 2-Dimethoxytrityloxymethyl-6-(3',6'-dipivaloylfluorescein-6-yl-carboxamido)-hexyl-1-O-succinoyl-long chain alkylamino CPG F.W.

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艾美捷Lumiprobe Universal CPG type II, 1000A 是一种通用支持物，用于固定核苷以合成寡核苷酸并在去封闭过程中提高 3' 末端寡核苷酸的去磷酸化速率。 对于从载体上的切割和寡核苷酸脱保护无水氨气相，可以在…

FAM-xtra is a new fluorophore that has the identical spectra to FAM, but is much brighter and more photostable than FAM. FAM-xtra CPG is used to add the significantly improved FAM label at the 3'-terminus in complimentary to our FAM-xtra Phosphoramidite that contains no 4,4'-dimethoxytrityl (DMT) group and can be added only once at the 5 ...