2022年6月1日 · The RT-qPCR was conducted to quantify the expressions of Fat1 and Ncl in Fasn -OE and control cells (Fig. 3). FAT1 and NCL were significantly upregulated under Fasn overexpression conditions, showing that Fasn positively regulated the expressions of FAT1 and NCL in islet cells.

2022年9月19日 · Fatty acid synthase (FASN) is an attractive therapeutic target in non-alcoholic steatohepatitis (NASH) because it drives de novo lipogenesis and mediates pro-inflammatory...

2022年4月1日 · We report here the identification of a group of type I interferon suppressed genes, including fatty acid synthase (FASN), which are involved in lipid metabolism. Overexpression of FASN or the addition of its downstream product, palmitate, increased viral infection while knockout or knockdown of FASN reduced infection.

2021年3月19日 · Fatty acid synthase (FASN) is the only human lipogenic enzyme available for de novo fatty acid synthesis and is often highly expressed in cancer cells. We found...

To determine the expression level of FASN, we analysed the cell lines by qRT-PCR (qPCR) and Western blotting. Despite different origins, all the CTCL cell lines had increased FASN expression compared to healthy PBMC at both the protein and mRNA level (Figure 1 A–C).

The reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used for validation of selected differentially expressed genes (DEGs) and Fasn-regulated alternative splicing genes (RASGs).

逆转录定量聚合酶链反应 (RT-qPCR) 用于验证选定的差异表达基因 (DEG) 和 Fasn 调节的可变剪接基因 (RASG)。 在这项研究中，我们发现 Fasn 过表达导致基因表达谱发生显着变化，包括免疫相关基因 mRNA 水平的下调，包括INS-1 细胞系中的 Bst2 、 Ddit3 、 Isg15 、 Mx2 、 Oas1a 、 Oasl 和 RT1-S3 . 此外，Fasn 正向调节转录因子如 Fat1 和 Ncl 糖尿病相关基因的表达。 重要的是，Fasn 过表达导致可变剪接事件，包括代谢相关的 ATP 结合蛋白 mRNA Abcc5。 在 Gene …

2024年8月1日 · Fatty acid biosynthesis and the expression of fatty acid synthase (FASN), a key enzyme catalyzing long-chain fatty acid synthesis, were significantly enriched in C. sinensis-related iCCAs. iCCA cell lines treated with excretory/secretory products of C. sinensis displayed elevated FASN and free fatty acids.

2021年1月25日 · Real-time quantitative PCR (RT-qPCR) and immunohistochemistry were performed to detect FASN expression in 380 paired ccRCC and normal tissues from the Fudan University Shanghai Cancer Center (FUSCC). Visceral adipose tissue (VAT) and subcutaneous adipose tissue were at the level of the umbilicus as measured by …

Mouse Fasn qPCR Primer Pair，即小鼠Fasn qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。