FITC (Fluorescein-5-isothiocyanate) is a fluorescent compound with an excitation peak at 491 nm and an emission peak at 516 nm. Other spectra of interest include: Cy5 (Cyanine-5), iFluor 488, and CFSE (fluorescein, CFDA, SE).

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the

Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated.

FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all JIR antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm.

Fluorescein (FITC) derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water-solubility. Fluorescein-based dyes and their conjugates have several performance characteristics that may facilitate or limit use in certain applications.

FITC是一种常用的绿色荧光探针，在激发后会发出绿色荧光信号，因此可以通过荧光显微镜、荧光酶标仪或流式细胞仪等进行检测。 FITC的吸收 (激发)和发射峰参见下表。 本Streptavidin-FITC用高纯度、高品质的Streptavidin和FITC偶联而成，确保低本底信号和高灵敏度。 本Streptavidin-FITC的浓度为1mg/ml，推荐的初始稀释浓度为1:50-200，实际实验操作过程中需根据具体情况适当调节Streptavidin-FITC的稀释比例。 -20ºC避光保存，一年有效。 4ºC避光保存，3个月有效。 …

Fitc is a chemical compound with an absorbance peak at 488 nm. AAT Bioquest's interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats. The Spectrum Viewer is set to fluorescent mode by default.

Fluorescein isothiocyanate (FITC) is a pH-sensitive fluorophore embedded in silica nanoparticles to measure the concentration of H+ ions in the environment by changing its fluorescence intensity ratio with a pH-insensitive fluorophore. You might find these chapters and articles relevant to this topic. Swathi Merugu, ... Gabriela Chiosis.

2009年5月22日 · We present the synthesis of fluorescein isothiocyanate (FITC)-labeled poly (ethylene oxide)- block -poly (ɛ-caprolactone)- block -poly (ethylene oxide) (PEO–PCL–PEO) triblock copolymers and their applications for tracking the penetration behavior of FITC-labeled copolymers in the hairless mouse skin.

FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm. Like most fluorochromes, it is prone to photobleaching.