Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Fluo-4 AM是最常用的检测细胞内钙离子浓度的荧光探针之一。 分子式为C 51 H 50 F 2 N 2 O 23，分子量为1096.95。 Fluo-4 AM在Fluo-3 AM的基础上改进而成，其特点是加载更快并且在相同条件下荧光更加明亮。 Fluo-4 AM将Fluo-3 AM结构中的氯原子替换成了氟原子，导致它的最大激发波长会向短波长处偏离12nm左右。 这个波长更接近于氩激光器的波长488nm，所以用氩激 …

BD Pharmingen™ Fluo-4 AM is a cell-permeant dye that can be used to measure the intracellular ionized calcium concentration, [Ca 2+]i. The hydrophobic acetomethoxy (AM) moiety allows passage across the cell membrane into viable cells.

Fluo-4 是 fluo-3 的类似物，其中两个氯取代基被氟取代，导致在 488 nm 波长处的荧光激发增强，因此荧光信号水平更高。 将溶解后的指示剂直接加入含有培养细胞的培养皿中，即可向细胞上样 AM 酯形式的这类钙离子指示剂。 这些指示剂适用于荧光和共聚焦显微镜、流式细胞分析和微孔板筛选应用。 此外，基于 BAPTA 的指示剂可结合多种重金属阳离子（例如 Mn 2+ 、Zn 2+ …

Intracellular Ca is measured by Fluo-4/AM (for 30 min) with flow cytometry. The cardiomyocytes are isolated by enzyme digestion and loaded with Fluo 4-AM (5 μM) at 37 °C for 20 min. …

Fluo-4 AM is a widely used fluorescent dye for measuring intracellular calcium (Ca) levels, a critical parameter in various physiological and pathological processes. The mechanism of Fluo-4 AM's operation involves several key steps, making it …

Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.

Fluo-4 AM is a high-sensitivity, cell-permeable calcium indicator dye that emits bright green fluorescence upon binding to Ca²⁺. Ideal for live cell imaging, microscopy, flow cytometry, and high-throughput screening, it detects intracellular calcium with low toxicity.

BD PharmingenTM Fluo-4 AM is a cell-permeant dye that can be used to measure the intracellular ionized calcium concentration, [Ca2+]i. The hydrophobic acetomethoxy (AM) moiety allows passage across the cell membrane into viable cells.

Fluo-4 AM is a cell-permeable calcium ion indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon calcium ion binding. Fluo-4 AM is well suited for fluorometric and imaging applications.