2020年3月1日 · FRET-based nucleic acid probes, which could signal the relative position changes of donor and acceptor molecules on account of cleavage, conformational changes and binding/unbinding events, are well-established with DNA backbones tagged with one or …

2019年8月30日 · FRET is non-radiative energy transfer that occurs by dipole–dipole coupling from an excited state donor (D) fluorophore to a ground state acceptor (A; another fluorophore or a...

FRET就是采用非放射方法，在 供体 和 受体 相互靠得很近（1-10 nm）时，将光子能从一个受激发的荧光团（供体）转移到另一个荧光团（受体）。 采用FRET，可以解决超过光学显微镜分辨率限制的分子相对邻近度问题，来显示（例如） CFP受光激发后便发射光 CFP受光激发后但没有发射小光。 CFP离YFP的距离大于10nm CFP与YFP非常接近（1-10 nm） YFP没有受到激发，因此也不发射光 CFP没有受光激发，但发射光. 受激发后的荧光团（供体）将受激发的静能转移到一 …

2024年11月11日 · In addition, the DNA nanocube SAM was configured to be used as a turn-on NeutrAvidin sensor using biotinylated DNA targets hybridized to each probe resulting in an increase in FRET. We show that the wireframe DNA nanocube can be an effective scaffold for preparing biosensors with controlled separation between surface-bound probes facilitating ...

2008年5月29日 · Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive...

荧光共振能量转移（fret)是一种非辐射能量跃迁，通过分子间的电偶极相互作用，将供体激发态能量转移到受体激发态的过程。 此过程没有光子的参与，所以是非辐射的。

Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochem …

2016年6月10日 · smFRET技术的原理就是将2个相互作用的分子标记上颜色不同的荧光基团，一个是在能量转移过程中提供能量，即供体D (donor);另一个接受能量，即受体A (acceptor)。 这样可以运用荧光共振能量转移 (fuorescence resonance energy transfer, FRET) 技术来对体系进行研究。 因此，比单个荧光基团标记更具有优势，称为单分子荧光共振能量转移 (single molecule fuorescence resonance energy transfer,smFRET) 技术。 令人欣喜的是，这项技术是由华人科 …

2006年5月24日 · FRET is a mechanism by which energy is transferred from an electronically excited donor molecule to a ground-state acceptor via through-space resonant dipolar coupling. The efficiency of this process is strongly distance dependent.

Fluorescence resonance energy transfer (FRET) is widely used in biomedical re-search as a reporter method. Oligonu-cleotides with a DNA backbone and one or several chromophore tags have found multi-ple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies.