2024年3月6日 · FTMT的基本信息如下： 1. 分子量：FTMT的分子量约为20 kDa。 2. 基因：FTMT的基因信息为FTMT，位于人类染色体5q23.2上。 3. 结构：FTMT是一种含铁蛋白，其结构与传统的铁蛋白相似，包含有铁传输蛋白的结构域和活性位点，能够与铁离子结合形成铁蛋白复合 …

Human mitochondrial ferritin (FtMt) was recently discovered and described as an intronless gene, found on chromosome 5q23.1, responsible for encoding a 242-amino acid precursor FtH-like protein. 33 FtMt is a 30-kDa protein, targeted to mitochondria and processed to a 22-kDa subunit that has ferroxidase activity similar to FtH.

To explore FtMt expression in human dopaminergic neurons, we designed a novel monoclonal antibody, C65-2, directed against human FtMt. Here, we report the properties of our C65-2 antibody. Western blots analysis and immunoabsorption tests demonstrated that the C65-2 antibody specifically recognized FtMt with no cross-reactivity to H-ferritin.

2020年9月25日 · Deferiprone, an iron chelator, increases the expression of mitochondrial ferritin (FTMT) through hypoxia inducible factor 1α‐specific protein 1 axis. FTMT specifically interacts with nuclear receptor coactivator 4, an autophagic cargo receptor. Deferiprone‐induced mitophagy occurs selectively for depolarized mitochondria.

FtMt protein was recognized as approximately 22 kDa by the anti-FtMt antibody (Fig. 1B, lane 4), but not by the anti-GFP antibody (Fig. 1B, lane 3), indicating the anti-FtMt antibody did...

2022年11月23日 · Among these proteins, the ferritin heavy chain is well characterized, but the regulatory principles of mitochondrial ferritin (FTMT) remain elusive. FTMT appears to be cleaved from a 27 kDa to a 22 kDa form. In human macrophages, FTMT increased under hypoxia in a hypoxia-inducible factor 2-dependent manner.

2017年5月1日 · In this study, we investigated the function of FtMt in α-synuclein regulation and its antioxidant roles in dopaminergic cells using human dopaminergic neuroblastoma cells, SH-SY5Y. In physiological conditions, FtMt knockdown increased α-synuclein expression at the protein level but not at the mRNA level.

2016年7月22日 · The 242-amino acid FtMt precursor protein is cleaved to mature protein (of molecular weight about 22-kDa) in the mitochondrial matrix. Compared with the ubiquitously expressed traditional ferritin (H-ferritin and L-ferritin), FtMt has been found in fewer locations including the testis, heart and brain.

FTMT appears to be cleaved from a 27 kDa to a 22 kDa form. In human macrophages, FTMT increased under hypoxia in a hypoxia-inducible factor 2-dependent manner. Occurrence of FTMT resulted from cleavage by thrombin, which was supplied by serum.

Western blot analysis of FTMT expression in HepG2 cell lysate. Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.