2019年1月28日 · Here, we report that purified FtsW polymerizes lipid II into peptidoglycan, but show that its polymerase activity requires complex formation with its partner class B PBP. We further demonstrate...

↑ 2.0 2.1 2.2 Khattar, MM et al. (1997) Two polypeptide products of the Escherichia coli cell division gene ftsW and a possible role for FtsW in FtsZ function. J. Bacteriol. 179 784-93 PubMed; ↑ Khattar, MM et al. (1994) Identification of FtsW and characterization of a new ftsW division mutant of Escherichia coli. J. Bacteriol. 176 7140-7 ...

The SAOUHSC_01063 [M1-N408] gene encoding FtsW was amplified from S. aureus RN4220 gDNA using primers oAT59 and oAT60. After digestion with SalI and BamHI, the PCR product was ligated into pLOW to produce pLOWftsW, which is an overexpression plasmid for SaftsW .

We used molecular and genetic techniques to introduce an insertional inactivation into the chromosomal copy of ftsW, in the presence of the plasmid-borne wild-type ftsW gene under the control of P(BAD).

The bacterial cell division protein FtsW has been suggested to perform two functions: stabilize the FtsZ cytokinetic ring, and facilitate septal peptidoglycan synthesis by the transpeptidase FtsI (penicillin-binding protein 3).

Here, we report that purified FtsW polymerizes lipid II into peptidoglycan, but show that its polymerase activity requires complex formation with its partner class B PBP. We further demonstrate that the polymerase activity of FtsW is required for its function in vivo.

FtsW, another essential cell division protein, was also localized to the septum. Many genes that are required for cell division have been identified in Escherichia coli. These include the fts genes ftsA, ftsZ, ftsQ, ftsN, ftsL, ftsK, ftsW, and ftsI and the recently identified zipA gene (21, 31).

Site-directed mutagenesis experiments combined with fluorescence microscopy shed light on the role of Escherichia coli FtsW, a membrane protein belonging to the SEDS family that is involved in peptidoglycan assembly during cell elongation, division, and sporulation. This essential cell division protein has 10 transmembrane segments (TMSs).

2011年4月4日 · Here, we show that the integral membrane protein FtsW, an essential protein of the bacterial division machinery, is a transporter of the lipid-linked peptidoglycan precursors across the cytoplasmic membrane.

2002年10月29日 · The membrane topology of Escherichia coli FtsW, a 46-kDa essential protein, was analyzed using a set of 28 ftsW–alkaline phosphatase (ftsW–phoA) and nine ftsW–β-lactamase (ftsW–bla) gene fusions obtained by in vivo and in vitro methods. The alkaline phosphatase activities or resistance pattern of cells expressing the FtsW–PhoA or ...