2020年8月17日 · By engineering the sequence of SmBiT, a series of peptides with various equilibrium dissociation constants were created, among which peptide 86 (HiBiT) …

2024年11月18日 · In brief, we used a Gα gustducin with a Renilla luciferase variant (RLuc8) inserted within the alpha-helical region along with an unlabelled beta subunit and a gamma …

HiBiT tag is a complimentary peptide (VSGWRLFKKIS), with high affinity for LgBiT. HiBiT tag is fused to the Gβ subunit of the receptor. The complementation of LgBiT and HiBiT facilitates …

2024年12月5日 · We co-expressed GPR119 with DNGα s and Gβγ in Sf9 insect cells and purified the complex using lauryl maltose neopentyl glycol (LMNG) and cholesterol hemisuccinate …

Gβ19, the NanoBiT tethering method can enhance the stability of the VIP1R–Gs complex and facilitate the structure study of this GPCR complex.

Here, we present the cryo-electron microscopy (cryo-EM) structure of the human H 1 R in complex with a G q protein in an active conformation via a NanoBiT tethering strategy.

Combined with Nb35, which is used to stabilize the complex between Gαs and Gβ 19 , the NanoBiT tethering method can enhance the stability of the VIP1R-Gs complex and facilitate …

2022年11月17日 · Adhesion G-protein-coupled receptors (aGPCRs) play key roles in a diversity of physiologies. A hallmark of aGPCR activation is the removal of the inhibitory GAIN domain …

2020年11月23日 · Base on structural complementation assay using NanoBiT technology, we characterized the distinct mechanisms underlying β-arrestin2 recruitment by both CXCR4 and …

2020年11月25日 · A bovine Gα s was used with G226A and A366S mutations to stabilize the complex; 43 rat Gβ1 was attached to HiBiT with a 15-amino acid linker between them; bovine …