病毒载体可以借助其他病原体的外壳蛋白包装成假病毒，以改变其嗜性，最常用的是疱疹性口腔炎病毒膜融合包膜G糖蛋白（VSV-G）。 VSV-G有两个明显的优势： (1) VSV-G蛋白比env蛋白更加稳定，因此通过VSV-G蛋白“假型化”的病毒可以进行超离浓缩，从而获得更高的滴度。 (2) VSV-G的受体是在细胞膜上广泛表达的磷脂酰丝氨酸，因此相较于env蛋白，VSV-G“假型化”病毒宿主范围变得更加广阔。 目前，绝大部分慢病毒载体均采用VSV-G作为包膜蛋白。 慢病毒包装系统- …

2024年12月4日 · Vesicular stomatitis virus (VSV) glycoprotein (G) is a prototype for class III fusion proteins. Although structures of the stable pre- and postfusion ectodomain of G are known, its fusogenic intermediates are insufficiently characterized.

2018年7月13日 · Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusion...

2025年2月12日 · Vesicular stomatitis virus glycoprotein G (VSV-G) is crucial in the viral life cycle, mediating entry into host cells and initiating infection. Understanding its structure and function is essential for developing antiviral strategies and enhancing gene therapy approaches.

This Delta-G-VSV Pseudotyping System has proven useful for identifying cellular receptors for viruses, screening for entry inhibitors, and evaluating neutralizing antibody responses following vaccination. Originally described in a 2010 methods article (PMID: 20709108), the system is now used as a virus model system by researchers worldwide ...

2013年1月31日 · Vesicular stomatitis virus G glycoprotein (VSV-G) is the most widely used envelope protein for retroviral and lentiviral vector pseudotyping; however, serum...

2025年1月9日 · Explore the structure and function of VSV-G glycoprotein and its innovative applications in gene therapy and vaccine development. The VSV-G glycoprotein is a component of the vesicular stomatitis virus, playing a role in viral …

2017年1月3日 · The glycoproteins (G proteins) of vesicular stomatitis virus (VSV) and related rhabdoviruses (e.g., rabies virus) mediate both cell attachment and membrane fusion. The reversibility of their fusogenic conformational transitions differentiates them from many other low-pH-induced viral fusion proteins …

This recombinant (rVSV-ΔG) has been used to produce VSV pseudotypes containing the envelope glycoproteins of heterologous viruses, including viruses that require high-level biocontainment; however, because the infectivity of rVSV-ΔG pseudotypes is restricted to a single round of replication the analysis can be performed using biosafety level ...

2025年1月9日 · The vesicular stomatitis virus (VSV) G protein is a key component of the viral envelope, essential for the virus’s ability to infect host cells. Its significance extends beyond basic virology, as it has become a focal point for numerous medical applications due to its unique properties and functionalities.