V H and V L are the variable domains. A single-domain antibody can be obtained by immunization of dromedaries, camels, llamas, alpacas or sharks with the desired antigen and subsequent isolation of the mRNA coding for the variable region (V NAR and V …

2023年5月24日 · 纳米抗体(nanobody,Nb)， 即重链单域抗体VHH (variable domain of heavy chain of heavy-chainantibody) 在骆驼科（2）的血清中发现了这种常规哺乳动物IgG结构的一个典型的例外。

IgG-VHH：重链优于轻链. sdAb包括纳米抗体（VHH），单独的重轻链可变区（VH/VL），monobody等，因为使用最多的是VHH，所以下文只讨论IgG-VHH。 2023年3月，研究者在mAbs杂志上发表文章，全面比较了基于IgG和VHH的双抗formats。

Here, we review the structural and biophysical properties of VHH domains, highlight recent VHH-based therapeutics and diagnostics, and provide insight into VHH engineering that may pave the way to next-generation single domain antibody applications.

The camelid-derived single chain antibody (sdAb), also termed VHH or nanobody, is a unique, functional heavy (H)-chain antibody (HCAb). In contrast to conventional antibodies, sdAb is a unique antibody fragment consisting of a heavy-chain variable ...

2023年8月19日 · We show that the CDR-H3 conformation of VHHs correlates with the germline from which the antibodies are derived: IGHV3-3 derived antibodies almost exclusively adopt a kinked CDR-H3 conformation...

2024年3月27日 · The recent exploration of a comprehensive panel of symmetric IgG-VHH bsAbs showed that fusion of VHH domains on IgG scaffolds is a robust way of forming functional and structurally diverse bsAbs with high thermodynamic stability and low aggregation propensity.

2017年11月25日 · Camelid single-domain antibodies (sdAbs, VHHs, or Nanobodies®) are types of antibody fragments that are composed of the heavy-chain variable domain only. These VHHs possess unique structural and functional features, as they are small in size and exhibit thermal stability and high solubility.

2020年2月11日 · Here, we report the characterization of a VHH-derived IgG-like bi- and trispecific antibody platform that essentially relies on the replacement of the VH and VL regions of a conventional antibody by two independently functioning VHH domains.

2022年10月27日 · Bispecific antibodies (bsAbs) represent a unique platform to increase antibody breadth and to reduce neutralization escape. Herein, a novel immunoglobulin G-variable domains of heavy-chain-only antibody (IgG-VHH) format bsAb derived from a potent human antibody R15-F7 and a humanized nanobody P14-F8-35 are rationally engineered.