2020年4月16日 · The central node of this network is G3BP1, which functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations. Moreover, we show that interplay between three distinct intrinsically disordered regions (IDRs) in G3BP1 regulates its intrinsic propensity for LLPS, and this is fine ...

2020年4月16日 · Biophysical studies revealed details of how G3BP1 functions as a molecular switch that triggers RNA-dependent LLPS. Importantly, interplay between three distinct intrinsically disordered regions (IDRs) in G3BP1 regulates its intrinsic propensity for LLPS, and this is fine-tuned by phosphorylation within the IDRs.

2022年1月5日 · Using high-resolution microscopy, we show that in resting cells, cGAS exhibits particle-like morphological characteristics, which are markedly weakened when G3BP1 is deleted. Upon DNA challenge, the pre-condensed cGAS undergoes liquid-liquid phase separation (LLPS) more efficiently.

2021年6月25日 · This rise in cytoplasmic mRNA triggers multicomponent liquid-liquid phase separation (LLPS) with RNA-binding proteins, creating a condensed liquid compartment that remains in dynamic equilibrium with, yet distinct from, the surrounding cytosol (3–5).

2021年8月17日 · G3BP1/2 interact with a large number of proteins, including Caprin-1 and TIA-1, and both of them promote G3BP1/2-mediated LLPS 2, 3. Very recently, G3BP1/2 is shown to interact with the...

2021年11月15日 · Here, we report that GTPase‐activating protein‐ (SH3 domain)–binding protein 1 (G3BP1) primes cGAS for its prompt activation by engaging cGAS in a primary liquid‐phase condensation state.

2024年12月1日 · When the concentration of free RNA within cells increases, it can trigger RNA-dependent liquid-liquid phase separation (LLPS) with G3BP1 as the core, thereby forming SGs that affect cell survival or death. In addition, G3BP1 interacts with various host proteins to regulate the expression of SGs.

2024年8月27日 · Liquid-liquid phase separation (LLPS) mediated by G3BP1/2 proteins and non-translating mRNAs mediates stress granule (SG) assembly. We investigated the phylogenetic evolution of G3BP orthologs from unicellular yeast to mammals and identified both conserved and divergent features.

2023年4月26日 · Mechanically, DNA-induced liquid-liquid phase separation (LLPS) of N protein competitively binds with G3BP1, a co-factor of cGAS, resulting in the disruption of cGAS-G3BP1 complex and...

2 天之前 · G3BP1 is a central node of the protein-RNA interaction network in SG formation, which functions as a molecular switch that triggers LLPS in response to the increase of intracellular free RNA concentrations . In previous studies, G3BP1 was found to act as an anchor for viral proteins to suppress SG formation (21, 22, 50). Notably, G3BP2 is a ...