Circular dichroism (CD) spectroscopy is a primary tool for the characterization of G-quadruplex (G4) structures. G-quadruplexes are functionally important genomic elements that form at specific locations in an orchestrated manner throughout the cell cycle.

2017年8月9日 · Circular dichroism (CD) has emerged as one of the standard biophysical techniques for the study of guanine-quadruplex (G4) folding, cation effect, and ligand binding. The utility of this technique is based on its robustness, ease of use, and requirement of only small quantities of nucleic acid.

2012年1月1日 · Circular dichroism (CD) is a widespread technique for studying the polymorphism of G-quadruplexes. In this chapter the CD spectral features characteristic of different folding topologies of G4-DNA are analyzed in terms of the sequence of the syn or anti glycosidic bond angle (GBA) within a quadruplex stem.

2017年8月9日 · 圆二色性（CD）已成为研究鸟嘌呤-四链体（G4）折叠，阳离子效应和配体结合的标准生物物理技术之一。 该技术的实用性是基于其坚固性，易用性和仅需少量核酸的需求。 该实验还可以扩展到教室，并适合跨学科，跨学科化学，生物化学和药物化学的研究生院的学生。 具体来说，学生采用可以采用G4构象的核酸溶液，在金属阳离子存在下进行退火，并通过特征性CD签名评估折叠和拓扑。 在课堂实验中，还可以通过更改阳离子类型来相互转换G4拓扑，并在不 …

2025年3月12日 · In this study we analyze and compare the CD patterns of two major groups of DNA sequences that form G4s and iMs: tandem repeats of GGGGCC/GGCCCC present in intron 1 of the C9ORF72 gene associated with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) and polyG/polyC-rich sequences present at the promoter-proximal regions of v...

Circular Dichroic (CD) spectroscopy is one of the most frequently used methods for guanine quadruplex studies and in general for studies of conformational properties of nucleic acids. The reason is its high sensitivity to even slight changes in …

2024年1月5日 · Circular dichroism (CD) is an effective technique for estimating the tertiary structure of nucleic acids at low concentrations under homogeneous conditions. This application note reports the tertiary structure evaluation of G-quadruplex (G4) aptamers using a high-throughput CD (HTCD) measurement system and the principal component analysis (PCA).

2005年11月28日 · View credits, reviews, tracks and shop for the 2005 CD release of "G4 & Friends" on Discogs.

Circular dichroism (CD) is a widespread technique for studying the polymorphism of G-quadruplexes. In this chapter the CD spectral features characteristic of different folding topologies of G4-DNA are analyzed in terms of the sequence of the syn or anti glycosidic bond angle (GBA) within a quadruple …

紫外可见吸收光谱（UV）、圆二色光谱（CD）方法具有样 品用量少、灵敏度高，检测速度快等优势，因而在 G－四链体 结构研究中起着举足轻重的作用。