
GAL4 - 百度百科
GAL4是酵母(Saccharomyces cerevisiae)转录激活蛋白Gal4的基因。Gal4能识别基因启动子的上游激活序列(UASg)的17bp长的一段序列:5'-CGGRNNRCYNYNYNCNCCG-3'(R表示 …
Gal4 transcription factor - Wikipedia
The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes. [1] This protein represents a large fungal family of transcription factors, Gal4 family, …
GAL4/UAS system - Wikipedia
The GAL4-UAS system is a biochemical method used to study gene expression and function in organisms such as the fruit fly. It is based on the finding by Hitoshi Kakidani and Mark …
酵母杂交那些事儿(一) - 知乎专栏
其基本思想是将感兴趣的两个蛋白x和y分别融合到gal4的dbd和ad上,这样x和y之间的相互作用可以重建gal4转录因子的功能,然后驱动报告基因的表达(图1)。在第一个诱饵结构中,蛋白 …
快问快答12| GAL4系统酵母双杂的原理 - 知乎 - 知乎专栏
问1. GAL4系统 酵母双杂交的实验原理是什么?. 答1:很多真核生物的位点特异转录激活因子(如GAL4、GCN4)通常具有两个可分割开的结构域,即 DNA特异结合域 (DNA-binding …
GAL4/UAS system - 简书
2024年6月27日 · 当gal4与uas结合时,uas序列会被激活,从而启动下游基因的转录。 工作原理 1. 构建两个转基因线. gal4驱动线:该线携带gal4基因,其表达受特定启动子控制。例如,假设 …
快问快答13|GAL4系统酵母双杂自激活怎么做? - 知乎专栏
2023年10月30日 · 自激活原理:一般情况下GAL4分子的BD可单独与上游激活化序列(upstream activating sequence,UAS)结合,但不能引起转录。 但是某些蛋白本身具有激活或转录功能, …
The GAL4 System - SpringerLink
The GAL4 system is a bipartite system in which one transgenic line, the driver, expresses GAL4 in a known temporal or spatial pattern and a second transgenic line, the responder, contains a …
GAL4/UAS Expression System - SpringerLink
2024年1月1日 · The GAL4/UAS System. There are many tools available when using the fruitfly, Drosophila melanogaster, a model organism highly amenable to genetic analysis.One …
Yeast Gal4: a transcriptional paradigm revisited - PMC
Abstract. During the past two decades, the yeast Gal4 protein has been used as a model for studying transcriptional activation in eukaryotes. Many of the properties of transcriptional …
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