2008年9月9日 · To accomplish this we used a recombinant adeno-associated virus vector, AAV1–glial fibrillary acidic protein (GFAP)–EAAT2, designed to selectively drive the overexpression of EAAT2 within astrocytes.

2008年9月9日 · To accomplish this we used a recombinant Adeno-associated virus vector, AAV1-GFAP-EAAT2, designed to selectively drive the overexpression of EAAT2 within astrocytes. Both viral mediated gene delivery and β-lactam antibiotic (penicillin-G) treatment of rat hippocampal slice cultures resulted in a significant increase in both the expression of ...

In this study, first, we found increased neuronal death and impairment of cognitive function in YAP GFAP-CKO mice (conditionally knock out Yes-associated protein [YAP] in astrocytes), and identified EAAT2 as a downstream target of YAP through RNA sequencing.

Excitatory amino acid transporter (EAAT) 1 and EAAT2 are Na + -dependant glutamate transporters expressed predominantly in glia cells of the central nervous system. As the most abundant glutamate transporters, their primary role is to modulate levels of glutamatergic excitability and prevent spill over of glutamate beyond the synapse.

Astrocytic EAAT2 Deficiency Results in Accelerated Age-Related Cognitive Decline. The EAAT2 gene was constitutively deleted by synapsin I promoter-driven Cre expression in neurons (syn-Cre + EAAT2 −/−) and through the tamoxifen-inducible human GFAP/Cre-ERT2 driver in …

2003年10月1日 · In this study we have generated transgenic mice expressing EAAT2 under the control of the GFAP promoter and then crossed these mice with the ALS-associated mutant SOD1(G93A) mice to investigate whether supplementation of the loss of EAAT2 that occurs during the disease progression would delay or rescue the disease progression.

2021年11月1日 · In this review we discuss targeting EAAT2, the predominant glutamate transporter in the CNS, as a promising approach for developing therapies for epilepsy. EAAT2 upregulation via transcriptional and translational regulation has proven successful in vivo in reducing spontaneous recurrent seizures and offering neuroprotective effects. Another ...

2012年10月25日 · In the present study we examined GFAP and excitatory amino acid transporter 2 (EAAT2) protein levels in the periaqueductal gray matter (PAG) after subacute and chronic restraint stresses to clarify changes in descending pain modulatory system in the rat with stress-induced hyperalgesia.

2008年9月9日 · To accomplish this we used a recombinant adeno-associated virus vector, AAV1-glial fibrillary acidic protein (GFAP)-EAAT2, designed to selectively drive the overexpression of EAAT2 within astrocytes.

The excitatory amino acid transporter 2 (EAAT2) is the dominant glutamatergic transporter in the human brain, responsible for efficient removal of glutamate from the synaptic cleft for recycling within glial cells. As such, it has a key role in maintaining excitatory-inhibitory homeostasis.