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The fluorescent screenable marker OLE1-GFP makes the transformed seeds fluorescent, thereby allowing the identification of transformed seeds immediately after harvesting seeds. Homozygous seeds can be identified in the T 2 seed population using OLE1-GFP.

2014年10月31日 · In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved...

To preserve germination ability, plant seeds must be protected from environmental stresses during the storage period. Here, we demonstrate that autophagy, an intracellular degradation system, maintains seed germination ability in Arabidopsis thaliana.

2021年6月25日 · To decipher the initiation and the mode of action of the endosperm-expressed LEC1, we closely monitored GFP signals in the developing seeds of PLL, PPL, and PPg from the zygote stage onward, to...

2018年6月19日 · Based on vacuole-targeted green fluorescent protein in Arabidopsis seeds, this method enables the nondestructive isolation of mutant seeds defective in vacuolar trafficking and their visual characterization.

Place the harvested F1 seeds under a fl uorescence dissection microscope and examine the seeds for GFP fl uorescence. The seeds can be broadly segregated into three categories: seeds...

In seeds at early developmental stages, PIN3-GFP was expressed in the whole ii1 layer with the cells just above the chalaza showing the highest signal intensity (Figure 7 a-f). ... View in full...

2024年12月5日 · In this work, we investigated the kinetic and structural effects of seeding on puncta and fibril formation using tau biosensor cells. We find that seeded cells produce fibrils with GFP immunolabeling. ImmunoEM of cell-purified fibrils showed GFP localized to the proteolytically sensitive fuzzy coat of tau fibrils.

We mutagenized transformant seeds expressing GFP-CT24. From approximately 3,000,000 lines of M2 seeds, we obtained >100 fluorescent seeds and designated them green fluorescent seed (gfs) mutants. We report 10 gfs mutants, all of which caused missorting of storage proteins.