2017年6月1日 · Using the spatial intensity distribution analysis (SpIDA), we found that the A206K mutation reduced the dimerization of GFP molecules. Further ectopic expression of Myristoyl …

近年来出现的新型wtgfp基因突变体的激发和发射谱发生了改变，热稳定性和荧光强度得到了提高，gfp报告基因在小鼠中的应用就是以这些变体作为基础的。 （4）增强型绿色荧光蛋 …

2015年7月9日 · In GFP-derived FPs, the A206K mutation robustly prevents dimerization at the hydrophobic interface of the β-barrel 21.

2012年8月1日 · The green fluorescent protein (GFP) from the jellyfish Aequoria victoria has been shown to dimerize at high concentrations, which could lead to artefacts in imaging …

Analysis of the fluorescence intensity distribution of GFP ( Figure 1 D) and GFP-A206K ( Figure 1 H) also showed different distributions in stoichiometry, and GFP had two distinct populations...

Plasmid TfRΔEctoR1-eGFP A206K from Dr. Jeanne Stachowiak's lab contains the insert TfRΔEctoR1-eGFP A206K and is published in Biophys J. 2018 Mar 27;114(6):1377-1388. doi: …

Enhanced GFP (EGFP) rendered monomeric by the A206K mutation.

Using the spatial intensity distribution analysis (SpIDA), we found that the A206K mutation reduced the dimerization of GFP molecules. Further ectopic expression of Myristoyl-GFP …

mEGFP is a basic (constitutively fluorescent) green fluorescent protein published in 2002, derived from Aequorea victoria. It has moderate acid sensitivity. mEGFP was derived from EGFP with …

We replaced hydrophobic residues at the crystallographic interface of the dimer with positively charged residues (A206K, L221K, or F223R). These mutants were likewise expressed and …