A green fluorescent protein (GFP)-derived double-stranded RNA (dsRNA-GFP) is currently commonly used as control in honey bee RNAi experiments, since its gene does not exist in the A. mellifera genome. Although dsRNA-GFP is not expected to trigger RNAi responses in treated bees, undesirable effects on gene expression, pigmentation or ...

2023年5月20日 · Nosema ceranae is an intracellular parasite invading the midgut of honeybees, which causes serious nosemosis implicated in honeybee colony losses worldwide. The core gut microbiota is...

2020年1月31日 · To test whether engineered S. alvi robustly colonizes bees, we inoculated newly emerged, antibiotic-treated bees en masse with S. alvi transformed with a plasmid expressing green fluorescent protein (GFP) and then monitored bacterial colonization .

We engineered a symbiotic bee gut bacterium, Snodgrassella alvi, to induce eukaryotic RNA interference (RNAi) immune responses. We show that engineered S. alvi can stably re-colonize bees and produce double-stranded RNA to activate RNAi and repress host gene expression, thereby altering bee physiology, behavior, and growth.

2022年12月2日 · Here the authors provide a protocol for functional genomics using engineered symbionts (FUGUES) that utilizes an engineered bee gut bacterium to induce a sustained RNAi response in the host that...

2013年1月4日 · Here, we performed three independent experiments using microarrays to examine the effect of dsRNA-GFP treatment (introduced by feeding) on global gene expression patterns in developing worker bees. Our data revealed that the expression of nearly 1,400 genes was altered in response to dsRNA-GFP, representing around 10% of known honey bee genes.

2017年7月25日 · We and others have used SINV-GFP to investigate honey bee 22, fruit fly 109, and mosquito 111 antiviral defense mechanisms, thus facilitating comparison of immune responses in both natural ...

Nonlethal monitoring with GFP allows continuous screening of virus growth and serves as a direct breeding tool for identifying honey bee parents with increased antiviral resistance.

2019年5月14日 · Here, we report on horizontal RNA flow among honey bees mediated by secretion and ingestion of worker and royal jelly diets. We demonstrate that transmission of jelly-secreted dsRNA to larvae is biologically active and triggers gene …

A green fluorescent protein (GFP)-derived double-stranded RNA (dsRNA-GFP) is currently commonly used as control in honey bee RNAi experiments, since its gene does not exist in the A. mellifera genome. Although dsRNA-GFP is not expected to trigger RNAi responses in treated bees, undesirable effects on gene expression, pigmentation or ...