This clone was derived from ATCC 25922 and contains a multicopy vector encoding the green fluorescent protein (Aequorea victoria) GFPmut3. This gene is expressed under the control of the P lac promoter (Escherichia coli). This construct has been designed for Gram-negative bacteria fluorescence labeling.

The use of green fluorescent protein (GFP) as a reporter for protein localization in Escherichia coli was explored by creating gene fusions between malE (encoding maltose-binding protein [MBP]) and a variant of gfp optimized for fluorescence in bacteria (GFPuv). These constructs encode hybrid proteins composed of GFP fused to the carboxy ...

2001年1月2日 · In this study, expression of green fluorescence protein (GFP) on the external surface of Escherichia coli was achieved by construction of a fusion protein between Lpp-OmpA hybrid and a GFP variant, GFP mut2.

2013年4月1日 · We ran PCR on protein coding DNA (GFP), cloned GFP into plasmids and transformed the plasmids into E. coli cell by varying the number of templates, and increased the denaturing time from 10 to 20 sec. We also ran reactions with primers designed for blunt and cohesive ends cloning, and “touch-down” PCR.

2006年10月24日 · Recombinant Escherichia coli (E. coli) bacteria expressing green fluorescent protein (GFP) was used as a model system to investigate the antimicrobial activities of Ag nanoparticles (NPs). A convenient in situ method of Ag NP synthesis using sodium borohydride, in the bacterial growth medium, was de …

2025年1月10日 · Explore the methods and applications of GFP gene cloning in E. coli, focusing on expression vectors, protein stability, and fluorescence detection. Green Fluorescent Protein (GFP) has become a vital tool in molecular biology due to its ability to emit bright green fluorescence when exposed to light.

In the protocol described in this article, ligation independent cloning into a GFP reporter vector is combined with fluorescence detection to rapidly screen the relative expression of multiple membrane proteins in E. coli. Vectors can be made in four working days with primary expression screening taking a further week.

The use of green fluorescent protein (GFP) as a reporter for protein localization in Escherichia coli was explored by creating gene fusions between malE (encoding maltose-binding protein [MBP]) and a variant of gfp optimized for fluorescence in bacteria (GFPuv).

2001年1月2日 · In this study, expression of green fluorescence protein (GFP) on the external surface of Escherichia coli was achieved by construction of a fusion protein between Lpp-OmpA hybrid and a GFP variant, GFPmut2.

2011年10月20日 · Skretas and Georgiou (2008) introduced random transposon insertion mutations into the E. coli strain MC4100A (ara +) chromosome, transformed the library with a plasmid encoding GFP-tagged human central cannabinoid receptor and isolated by FACS bacteria with enhanced GFP fluorescence. This led to the identification of a detrimental effect of ...