A brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP ...

1994年2月11日 · A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expressio …

Osamu Shimomura painstakingly isolated GFP from hundreds of thousands of jellyfish, characterized the chromophore and elucidated the mechanism of Aequorean bioluminescence. Martin Chalfie expressed the protein in E. coli and C. elegans, and Roger Tsien developed a palette of fluorescent proteins that could be used in a myriad of applications.

In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a …

2008年10月8日 · Roger Tsien and co-workers explained how three amino acids in the peptide backbone of GFP — namely serine, tyrosine and glycine in positions 65, 66 and 67, respectively — react in the presence of...

This GFP revolution in the biological sciences has been greatly accelerated by a rapid parallel development of quan-titative light microscopy, electronics, computational power and molecular modelling of intra- and inter-cellular processes with systems-biology approaches.

2010年2月3日 · Martin Chalfie demonstrated the use of GFP as a luminescent reporter gene. In one of his pioneering experiments, he made six individual cells fluoresce by expressing GFP in the transparent roundworm C. elegans. Roger Y. Tsien contributed to a better understanding of the mechanism of GFP fluorescence.

2009年10月1日 · This demonstration of GFP as a tool to study proteins in vivo fundamentally altered the nature and scope of the issues that could be addressed by cell biologists.

GFP is found in a jellyfish that lives in the cold waters of the north Pacific. The jellyfish contains a bioluminescent protein–aequorin–that emits blue light. GFP converts it to green light which we see when the jellyfish lights up. The GFP shown here and used as a base for paper model is from PDB structure 1ema.

2016年1月15日 · Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from diverse marine animals are widely used in biological science as a universal...