Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) enzymes (EC 1.2.1.12) are a family of abundantly expressed oxidoreductases known for their role in glucose metabolism. Research on the role of mammalian GAPDH in various biological signaling pathways over the last couple of decades has increased interest in this classical glycolytic enzyme.

2024年8月12日 · One such protein is glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the saliva of the Recilia dorsalis (RdGAPDH) leafhopper, which is known to transmit rice gall dwarf virus (RGDV). Here we...

方法在过氧化氢和还原型谷胱甘肽（gsh）存在下，兔肌肉gapdh被s-谷胱甘肽化。 通过MALDI-MS分析，差示扫描量热法，动态光散射和超速离心法测定修饰的蛋白质。

2017年12月1日 · Rabbit muscle GAPDH was S-glutathionylated in the presence of H 2 O 2 and reduced glutathione (GSH). The modified protein was assayed by MALDI-MS analysis, differential scanning calorimetry, dynamic light scattering, and ultracentrifugation.

2020年6月1日 · The goal of the present work was to obtain human recombinant wild-type GAPDH (hGAPDH) and GAPDH with C156S substitution (hGAPDH_C156S), to compare structural changes caused by S-glutathionylation of these proteins, and to study the ability of the modified proteins to reactivate in the presence of GSH, glutaredoxin and thioredoxin.

2023年4月6日 · Here we show that human and mouse cells expressing a GAPDH mutant lacking this redox switch retain catalytic activity but are unable to stimulate the oxidative pentose phosphate pathway and...

2010年10月24日 · Our findings reveal a novel mechanism for targeted nitrosylation of nuclear proteins and suggest that protein–protein transfer of nitric oxide groups may be a general mechanism in cellular signal...

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a key enzyme of glycolysis, is sensitive to oxidative modifications and involved in apoptotic signaling via the SirT1/p53 pathway in the nucleus. We aimed to elucidate the extent to which S -glutathionylation of GAPDH and glutaredoxin-1 contribute to GAPDH/SirT1/p53 apoptosis pathway.

Methods: Rabbit muscle GAPDH was S-glutathionylated in the presence of H 2 O 2 and reduced glutathione (GSH). The modified protein was assayed by MALDI-MS analysis, differential scanning calorimetry, dynamic light scattering, and ultracentrifugation.

2020年1月1日 · 半胱氨酸亚磺酸与还原型谷胱甘肽（gsh）反应形成混合的二硫键（s-谷胱甘肽化的gapdh），该二硫键进一步与cys154反应，在酶的活性位点产生二硫键。 与亚磺酸和磺酸相反，混合的二硫键和分子内的二硫键是可逆的氧化产物，可以在存在GSH或硫氧还蛋白的情况下 ...