2011年2月11日 · In the absence of the GadY RNA, a longer mRNA encompassing both cat and gfp was detected, while expression of GadY resulted in a smaller transcript (~ 0.9 kb) encompassing only gfp (Fig. 1 f). These results suggest that expression of GadY causes a processing event in the mRNA intergenic region between the two reporter genes.

Given that IS183 is encoded in the gad gene cluster and because of its role in regulating acid response genes reported here, this RNA has been renamed GadY. We show that GadY exists in three forms, a long form consisting of 105 nucleotides and two processed forms, consisting of 90 and 59 nucleotides.

Given that IS183 is encoded in the gad gene cluster and because of its role in regulating acid response genes reported here, this RNA has been renamed GadY. We show that GadY exists in three forms, a long form consisting of 105 nucleotides and two processed forms, consisting of 90 and 59 nucleotides.

接下来，转录组测序和qPCR揭示AR2系统的关键成员谷氨酸脱羧酶W（GadW）和GadY的表达受SdiA调控。 此外，β-半乳糖苷酶报告基因检测显示W 和 快乐受到SdiA的积极调控。

2020年6月2日 · Next, transcriptome sequencing and qPCR disclosed that the expression of glutamate decarboxylase W (GadW) and GadY, the key members of the AR2 system, were regulated by SdiA. Further, β-galactosidase reporter assays showed that the promoter activity of gadW and gadY was positively regulated by SdiA.

研究发现 GadY 通过与其目标 mRNA SdiA 碱基配对来促进 SM10λπ-PAO1 缀合，SdiA 是一种孤儿 LuxR 型受体，对外源 N-酰化高丝氨酸内酯 (AHL) 做出反应。 然而，由于 EC600 缺乏 AHLs 合酶，SM10λπ-EC600 缀合并未受到影响。 这表明 GadY 对缀合的影响取决于 AHLs-SdiA 信号传导。

2011年2月11日 · We previously showed that the small RNA GadY positively regulates gadX mRNA levels. The gadY gene is located directly downstream of the gadX coding sequence on the opposite strand of the chromosome. We now report that gadX is transcribed in an operon with gadW, although this full-length mRNA does not accumulate.

2004年11月1日 · Given that IS183 is encoded in the gad gene cluster and because of its role in regulating acid response genes reported here, this RNA has been renamed GadY. We show that GadY exists in three...

GadY RNA (previously named IS183 in [1]) is a non-coding RNA. The GadY gene is located on between and on the opposite strand [clarification needed] to the GadX and GadW genes.

2015年9月18日 · In this study, constitutive expression of the sRNA GadY was evaluated as a possible regulator of acid resistance in E. coli grown at high glucose concentration to high density. GadY was selected since the three forms of GadY (GadY 105, GadY 90, and GadY 59) were expressed when E. coli was grown at pH 6.0 in shake flasks [ 15 ].