2020年4月28日 · In a first set of studies, mononuclear CB cells were classified based on their CD18 expression as CD18 high and CD18 low/neg (gating strategy Fig. S1A). In each of these two populations, the content of CD34 + , CD34 + CD38 − , CD34 + CD38 − CD45RA − , and CD34 + CD38 − CD45RA − CD90 + was analyzed.

2024年12月4日 · The top two samples (ID430 and ID437) and the pooled library were compared in this paper. For both human and NHP villi studies, the first layer includes 7 major populations (CD14− innate, monocytes, NKT cells, CD38- B cells, CD8 T cells, CD4 T cells, and CD38+ B cells) gated by six markers (CD18, CD8a, CD38, CD14, CD3, and CD4).

To improve the monocyte purity, we devised a new gating scheme that takes advantage of the shared coexpression of cell surface markers on each subset. In addition to CD14 and CD16, CCR2, CD36, HLA-DR and CD11c are the most informative markers that discriminate among the three monocyte populations.

In summary, we demonstrated that: (i) CD11A/CD18 is constitutively overexpressed in tri12 CLL and correlates with ITGB2 promoter hypomethylation; (ii) an activating microenvironment up-regulates CD11A/CD18 in both non-tri12 and tri12 CLL cells via a DNA methylation-independent process; (iii) tri12 CLL hereby display a higher propensity to ...

2021年10月19日 · These heterodimeric adhesion molecules consist of a common βsubunit (CD18) that associates with one of four αsubunits: CD11a (LFA1), CD11b (MAC1), CD11c (p150), or CD11d. 15 Based on their amino-acid homology, CD11b and CD11c are quite similar proteins, with close to 50% amino-acid similarity.

2015年5月1日 · We examined the role for T cell-extrinsic CD18 in the activation of WT CD4 + T cells in vivo through the adoptive transfer of DO11.10 Ag-specific CD4 + T cells into CD18 −/− mice. We found that T cell-extrinsic CD18 was required for attenuating OVA-induced T cell proliferation in peripheral lymph nodes (PLN).

2020年4月28日 · These studies examined the function of CD18 in regulating neutrophil production and determined whether the defects in neutrophil production that are observed in CD18 deficiency persist in the...

1990年12月15日 · Correllated expression of CD11a and CD11c is shown for those cells which do not express CD3, CD16, CD19, or glyco; gating for this subset is shown in the inset, the histogram for which was generated by staining cells with a “cocktail” of FITC-conjugated MoAbs to CD3.

"Gating" refers to the selection of successive subpopulations of cells for analysis in flow cytometry. It is usually performed manually, based on expert knowledge of cell characteristics. However, there can be considerable disagreement in how gates should be applied, even between individuals experie …

Therefore, we pretreated wild-type and CD18-deficient murine neutrophils with GAME-46, a blocking antibody specific to CD18, and performed bacterial phagocytosis and binding/association assays. Functional blocking of CD18 in wild-type neutrophils resulted in diminished uptake of PA14 WT and PAO1 WT ( Fig. 6A and andB), B ), which phenocopied ...