When bound to Ca 2+, GCaMP fluoresces green with a peak excitation wavelength of 480 nm and a peak emission wavelength of 510 nm. [3] It is used in biological research to measure …

GCaMP6f is a multistate uv fluorescent protein published in 2013, derived from Aequorea victoria.

2023年3月15日 · Using large-scale screening and structure-guided mutagenesis, fast and sensitive GCaMP sensors are developed and optimized with improved kinetics without …

2023年8月28日 · The extinction coefficient measures how effectively GCaMP absorbs light of a particular wavelength, which affects its ability to detect changes in the intracellular Ca2+ …

For example, GCaMP excitation is blue (~470nm) and emission is green (~530nm). And this is where the second component is necessary. Dichroics and filters allow for the transmission of …

GCaMP is a genetically encoded calcium indicator used in neuroscience research, consisting of a fusion protein of green fluorescent protein (GFP) and calmodulin. It increases fluorescence …

2019年6月17日 · We present enhanced GCaMP sensors tailored to detect neuronal activity in specific applications. jGCaMP7 indicators include: jGCaMP7s (sensitive and slow), jGCaMP7f …

The pH titration fluorescence measurements are wavelength dependent. Using a more red shifted excitation wavelength for the pH-dependent fluorescence measurements (488 nm versus 470 …

By using one of the two beams as a reference and varying the wavelength of the second beam, we are able to determine precisely the wavelength dependence of ΔF/F in a live mouse brain. …

2023年2月22日 · We review the principles of development and deployment of genetically encoded calcium indicators (GECIs) for the detection of neural activity. Our focus is on the popular …