GCaMP is a genetically encoded calcium indicator (GECI) initially developed in 2001 by Junichi Nakai. [1] . It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and …

GCaMP由绿色荧光蛋白（green fluorescent protein , GFP）、钙调蛋白（calmodulin, CaM）和肌球蛋白轻链激酶的一段肽段序列M13构成。 当有Ca2+结合CaM时，CaM发生构象变化，其轻 …

2012年10月3日 · By combining in vivo imaging with electrophysiology we show that GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor …

Plasmid G-CaMP3 from Dr. Loren Looger's lab contains the insert G-CaMP3 and is published in Nat Methods. 2009 Dec;6 (12):875-81. This plasmid is available through Addgene.

2014年8月27日 · In addition, these novel transgenic parasites, PfGCaMP3, is a useful tool for screening and identifying new classes of compounds with anti-malarial activity. This …

2023年3月15日 · In particular, the green fluorescent protein (GFP)-based GCaMP sensors 2, 3, 5, 6 have been iteratively engineered to enhance the signal-to-noise ratio (SNR) for detecting Ca …

2014年1月1日 · It provides a novel method for imaging calcium fluctuations in the cytosol of P. falciparum, without signal interference from the host cell and invasive loading protocols. This …

2009年11月8日 · GCaMP3 is more photostable than the FRET indicators D3cpV and TN-XXL and had much greater sensitivity and faster kinetics, especially at higher levels of activity. GCaMP3 …

2013年7月18日 · Using GCaMP3 as a starting scaffold, we developed a library of GCaMP variants with a range of affinities and response rates. We found that our variants termed Fast …

2012年10月3日 · Signal-to-noise ratio was improved by at least 2- to 3-fold. In the visual cortex, two GCaMP5 variants detected twice as many visual stimulus-responsive cells as GCaMP3. …