
Fast and sensitive GCaMP calcium indicators for imaging neural
2023年3月15日 · The GCaMP6 (ref. 5) and jGCaMP7 (ref. 6) sensors enable detection of single action potentials (APs) under favourable conditions and are often used to monitor the activity of large groups of ...
Ultrasensitive fluorescent proteins for imaging neuronal activity
2013年7月17日 · Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium...
GCaMP6f :: Fluorescent Protein Database
GCaMP6f is a multistate uv fluorescent protein published in 2013, derived from Aequorea victoria.
Structural basis of the ultrasensitive calcium indicator GCaMP6
The newly developed GCaMP6 shows superior brightness and ultrasensitivity to calcium concentration change. In this study, we determined crystal structures of Ca(2+)-bound GCaMP6 monomer and dimer and presented detailed structural analyses in comparison with its parent version GCaMP5G.
Imaging neuronal activity in the central and ... - PubMed Central …
The genetically encoded calcium (Ca2+) sensor GCaMP6 has been widely used for imaging Ca2+ transients in neuronal somata, dendrites, and synapses. Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the ...
Deciphering the molecular mechanism responsible for …
Our detailed spectroscopic study reveals the simplest explanation for how GCaMP6m changes fluorescence in response to Ca 2+ is with a four-state model, in which a Ca 2+ -dependent change of the chromophore protonation state, due to a shift in pK a, is the predominant factor.
Ultrasensitive fluorescent proteins for imaging neuronal activity
2013年7月18日 · Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo.
In vivo measurement of afferent activity with axon-specific calcium ...
2018年8月20日 · Here we developed an active transportation strategy to enrich GCaMP6, a genetically encoded calcium indicator, uniformly in axons with sufficient brightness, signal-to-noise ratio, and...
Genetic Reporters of Neuronal Activity: c-Fos and G-CaMP6
Two such alternative methods with cellular scale resolution have matured in the last few decades and will be reviewed here: the transcription of immediate early genes, foremost c-fos, and the influx of calcium into neurons as reported by genetically …
Genetic reporters of neuronal activity: c-Fos and G-CaMP6
GCaMP6 has several isoforms that trade off temporal resolution for signal-to-noise, but the fastest are capable of resolving individual action potentials, provided the microscope used scans quickly enough.
- 某些结果已被删除