2020年7月23日 · Using translation elongation inhibitors and general cellular stress conditions, including amino acid starvation and UV irradiation, we show that ribosome collisions activate the stress-activated protein kinase (SAPK) and GCN2-mediated stress response pathways.

2024年12月25日 · GCN1 (GCN1 Activator Of EIF2AK4) is a Protein Coding gene. Diseases associated with GCN1 include Ogden Syndrome and Pulmonary Interstitial Emphysema. Among its related pathways are Cellular responses to stimuli and Peptide chain elongation. An important paralog of this gene is ECPAS.

2020年4月23日 · GCN1 is a GCN2-binding protein that possesses an RWD binding domain (RWDBD) in its C-terminus. In yeast, Gcn1 is essential for Gcn2 activation by AAS; however, the roles of GCN1 in mammals need to be established.

GCN1是一种GCN2结合蛋白，在其C端具有RWD结合域（RWDBD）。 在酵母中，Gcn1对通过AAS激活Gcn2至关重要。 但是，需要确定GCN1在哺乳动物中的作用。 在这里，我们通过产生两个Gcn1突变小鼠系揭示了不依赖AAS的GCN1的新作用：Gcn1基因敲除小鼠（Gcn1 KO小鼠（Gcn1-/-））和RWDBD缺失的突变小鼠（Gcn1ΔRWDBD小鼠）。 两只突变小鼠均表现出生长迟缓，这在Gcn2 KO小鼠中未观察到，因此Gcn1 KO小鼠由于严重的生长迟缓而在胚胎发育的 …

2024年6月1日 · As an upstream activator of protein kinase GCN2, GCN1 plays a pivotal role in integrated stress responses, such as amino acid starvation and oxidative stress. Through interaction with GCN2, GCN1 facilitates the activation of GCN2, thus initiating downstream signaling cascades in response to cellular stressors.

The Gcn1/Gcn20 protein complex binds to the GCN2 N-terminus, and this binding is required for efficient GCN2 activation in vivo (Garcia-Barrio et al., 2000). GCN1 is subject to negative regulation by yeast Yih1 (mammalian IMPACT), which prevents GCN1 binding to GCN2, thus indirectly inhibiting GCN2 ( Pereira et al., 2005 ).

The serine/threonine-protein kinase general control nonderepressible 2 (GCN2) is a well-known stress sensor that responds to amino acid starvation and other stresses, making it critical to the maintenance of cellular and organismal homeostasis.

2020年4月23日 · GCN1 is a GCN2-binding protein that possesses an RWD binding domain (RWDBD) in its C-terminus. In yeast, Gcn1 is essential for Gcn2 activation by AAS; however, the roles of GCN1 in mammals need to be established.

2024年5月4日 · 作为蛋白激酶 gcn2 的上游激活剂，gcn1 在氨基酸饥饿和氧化应激等综合应激反应中起关键作用。通过与 gcn2 相互作用，gcn1 促进 gcn2 的激活，从而启动响应细胞应激源的下游信号级联反应。在这些情况下，gcn2 的激活需要 gcn1 的存在和作用。

2000年4月17日 · We found that the GCN1-GCN20 complex physically interacts with GCN2, binding to the N-terminus of the protein. Overexpression of N-terminal GCN2 segments had a dominant-negative phenotype that correlated with their ability to interact with GCN1-GCN20 and impede association between GCN1 and native GCN2.