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Gfgh - YouTube
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Recombinant goldfish growth hormones (gfGH-I and -II) …
2003年8月1日 · Growth hormone (GH) is a polypeptide of approximately 22 kDa produced from somatotrophs in the anterior pituitary gland for the control of normal growth, development (Nicoll et al., 1999) and metabolism (Kaplan, 1999) in all vertebrates, and they were also shown to promote seawater adaptation in salmonid species (Bolton et al., 1987, Collie et a...
Recombinant goldfish growth hormones (gfGH-I and -II) …
The recombinant gfGH-I (five cysteines) and -II (four cysteines) were produced in Escherichia coli as the fusion proteins carrying N-terminal 6XHis tag, which facilitates purification by using metal chelating affinity chromatography under denaturing condition with urea.
Production of a biologically active novel goldfish growth hormone …
1998年8月1日 · Goldfish pituitary contains two types of growth hormones. One with five cysteine residues (type-I) similar to other Cyprinid GHs, and the other with four Cys residues (type-II) similar to those of other fish and tertapod species. Recombinant goldfish type II GH (gfGH-II) was produced in Escherichia coli using the pRSETB expression vector.
Study of goldfish (Carassius auratus) growth hormone structure ... - PubMed
Using goldfish as a model, the structure-function relationship of goldfish growth hormone was studied using the strategy of homologous domain swapping. Chimeric mutants were constructed by exchanging homologous regions between goldfish growth hormone (gfGH II) and goldfish prolactin (gfPRL) with the …
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中兴 C600 万兆OLT设备板卡 EFTL/GFGH/GFXH/GFCH - 百度贴吧
怎么区分板卡是千兆口还是万兆口? 就是看板卡型号吗? 还是有什么命令可以查,show什么?
Production of a biologically active novel goldfish growth hormone …
Recombinant goldfish type II GH (gfGH-II) was produced in Escherichia coli using the pRSETB expression vector. The gfGH-II was produced fused to a leader sequence, which sequestered into inclusion bodies after expression.