2023年8月1日 · Giant unilamellar vesicles (GUVs) are cell-membrane mimetic tools. GUV preparation techniques and formation mechanisms are discussed that determine vesicle properties. Immobilization strategies of GUVs are summarized. Key membrane-biophysics and bottom-up biology applications of GUVs are highlighted.

2013年11月5日 · We have succeeded in controlling tubular membrane formations in binary giant unilamellar vesicles (GUVs) using a simple temperature changing between the homogeneous one-phase region and the two-phase coexistence region. The binary GUV is composed of inverse-cone (bulky hydrocarbon chains and a small headgroup) and cylinder-shaped lipids.

GUV + kinesins + microtubules: Roux et al. showed that motor proteins that bind to the membrane pull a tube after getting load support from the microtubules. Leduc and colleagues found that these molecular motors are able to pull membrane tubes and tube formation depends on both motor protein density and membrane tension.

2018年10月1日 · Giant unilamellar vesicles (GUVs) are large vesicles bounded by a single lipid bilayer, which have been used in various applications as artificial, cell-like compartments. The water-in-oil (w/o) emulsion-transfer method has been attracting attention as a method to prepare GUVs that can efficiently encapsulate macromolecules.

Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane mimetic platforms. Their applications range from understanding and quantifying membrane biophysical processes to acting as elementary blocks in the bottom-up assembly of synthetic cells.

巨型单层囊泡（giant unilamellar vesicles，GUVs），指的是和细胞差不多大小的脂质泡，其外表的脂膜可以用来模拟细胞膜，也可以称之为仿生囊泡，能用于人造细胞方面的研究，那是个“高级”研究，不过本文要说的是更常见的药物筛选领域。 （用微流控技术生成细胞大小囊泡示意图） 传统方法做的囊泡，往其脂膜添加一些生物分子（如Min蛋白）后，会导致囊泡破裂。 但在2016年，科学家基于微流控技术已将其很好地攻克，使用的方法叫OAL法（辛醇辅助脂质体组装方 …

Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane mimetic platforms. Their applications range from understanding and quantifying...

In this review, we focus on a preparation technique for GUVs that encapsulate water-soluble materials and/or water-dispersible particles. It is based on the centrifugation of a water-in-oil emulsion layered on water and does not require special equipment other than a centrifuge, which makes it the first choice for laboratory use.

In this review, we describe an application of the single GUV method to studies on interactions of peptides with lipid membranes, especially interac-tion of antimicrobial peptide magainin 2 with lipid membranes. We show new valuable information on the magainin 2–induced leakage of internal contents of liposomes and pore formation in lipid membranes.

2018年4月16日 · The GUV membrane can be designed suitably to present selected, correctly-oriented cell-membrane proteins, whose mobility is confined to two dimensions. Here, we present recent advances in GUV design and the use of GUVs as cell models that enable quant. testing leading to insight into the working of real cells.