2025年1月15日 · Knocking out the enzyme gamma glutamyl carboxylase (GGCX) reduces virus replication in vitro and in vivo by inhibiting the carboxylation modification of viral haemagglutinin (HA) and the adhesion...

2024年10月31日 · Intercellular cross-talk analysis highlighted enhanced interactions among bystander immune cells during H1N1 infection, with neutrophils in humans and macrophages in mice showing the most remarkable increases in interaction intensity.

2021年9月14日 · In this study, we show that glycolysis is upregulated in H1N1-infected human lung epithelial (A549) cells and mouse lung tissues and that inhibiting this metabolic pathway results in reduced H1N1 replication. Furthermore, the effect of glycolytic inhibitors or enhancer on H1N1 infection is independent of interferon signaling.

2024年2月1日 · HIF-1α is one of the major host molecules that facilitates H1N1 replication and promotes cytokine storm. Mortality was decreased in HIF-1α knockdown mice infected with H1N1 compared to wild-type H1N1-infected mice. This study explores the mechanism of HIF-1α, which regulates glucose metabolism reprogramming in alveolar epithelial cells.

Ruan et al. show that H1N1 influenza A virus cross-activates Gli1, a transcription factor in the sonic hedgehog pathway. Gli1 activation plays a role in downregulating the expression of intercellular junction proteins, increasing the permeability of the alveolar epithelial barrier, and promoting inflammation in the lung tissue.

2 天之前 · Manivasagam et al. describe a novel mechanism by which the evolutionarily conserved CIC-ATXN1L transcriptional repressor complex regulates IFN and ISGs under both homeostatic and viral infection conditions. The CIC-ATXN1L complex binds to CBS motifs near ISG promoters to repress transcription, which is removed through …

2025年2月21日 · Wagoner and Yates et al. leverage tonsil organoids to explain heterogeneity in human immune responses to influenza vaccination. They identify novel predictors (Th1 signatures) of enhanced neutralizing antibody responses and validate this finding using in vivo data, demonstrating the utility of their model for identifying novel correlates of protection.

2024年3月1日 · In the current study, human memory (M) and naïve (N) T cells responses in mononuclear cells of tonsil (TMCs) and peripheral blood (PBMCs) were stimulated by pH1N1/sH1N1, and then stained for estimation of T cells proliferation index.

2 天之前 · We infected human nasal epithelial cells (hNECs) cultured at the air-liquid interface from multiple healthy donors with clinical isolates of major human seasonal influenza viruses (H1N1, H3N2, influenza B) and HPAI H5N1. The infected cells were subjected to virologic, transcriptomic and secretory protein analyses.

2019年3月14日 · In this study, we identified a fully human H1N1 neutralizing antibody (32D6) via an Epstein-Barr virus-immortalized B cell-based technology. 32D6 specifically neutralizes the clinically...