2018年7月11日 · Here we analyze both evolutionary rates and positive selection of the head and stalk domains of HAs from pre-pandemic seasonal H1N1 (sH1N1), pandemic H1N1 (pH1N1), H3N2 and the two...

The head-directed mAb, targeting an epitope distinct from known head-specific mAbs, showed relatively broad H1N1 neutralization and the stem-directed mAb was able to broadly neutralize diverse H1N1 viruses.

2019年4月3日 · Here, we quantified H1N1 HA head- and stalk-specific Abs in 179 adults hospitalized during the 2015-2016 influenza virus season. We found that HA head Abs, as measured by hemagglutinin inhibition (HAI) assays, were associated with protection against naturally acquired H1N1 infection.

Taken together, these data demonstrate that the anti-HA stalk antibodies boosted by vaccination with 1976 and 2009 H1N1 viruses correspond to an enhanced capacity to neutralize virus harboring a homologous HA stalk and a heterosubtypic HA head domain.

2010年7月12日 · Here, we describe the properties of two novel H1N1 swine-like HA proteins, HA1 (1–330) and HA (1–480), expressed in E. coli. Both proteins were properly folded as determined by CD spectra, binding to post-infection and post-vaccination sera, and could adsorbed neutralizing activity from H1N1 immune sera.

2010年7月12日 · In this study, two forms of HA proteins derived from the currently circulating novel H1N1 A/California/07/2009 virus, HA1 (1-330) and HA (1-480), were expressed and purified from E. coli under controlled redox refolding conditions that favoured proper protein folding.

2021年6月2日 · Here, we reveal that first exposure to the 2009 pandemic H1N1 influenza virus recalls memory B cells that are specific to the conserved receptor-binding site (RBS) or lateral patch epitopes of the hemagglutinin (HA) head domain.

2016年4月18日 · We developed a novel influenza A hemagglutinin-based display platform for H1 hemagglutinin stalk peptides that we identified in an epitope mapping assay using human immune sera and synthetic HA peptides.

2023年4月28日 · In the present study, we characterised the sequence and structure of HA from the H1N1 influenza strain A/Denver/57 bound to the bnAb C05 Fab and compared it to six additional H1N1 IVA HAs.

2021年7月2日 · The head-directed mAb, targeting an epitope distinct from known head-specific mAbs, showed relatively broad H1N1 neutralization and the stem-directed mAb was able to broadly neutralize diverse H1N1 viruses.