Successful Anti-PD-1 Cancer Immunotherapy Requires T Cell …
2018年12月18日 · We tracked IFN-γ and IL-12p40 in vivo during rejection of aPD-1 treatment-sensitive MC38 tumor cells, which were labeled with H2B-mApple. We also tracked …
Cell fusion potentiates tumor heterogeneity and reveals …
Stable cancer cell lines, MC38 (H2B-RFP), MC38 (H2B-RFP/Cre, B16F10 (H2B-RFP), and B16F10 (H2B-RFP/Cre), were generated by retroviral transduction using pBABE-based …
Arg1 expression defines immunosuppressive subsets of tumor …
MC38 cancer cells expressing H2B-mApple were implanted into the reporter mice, and tumors and other organs were harvested after 7 days. Animal tissues were subsequently analyzed …
TLR7/8-agonist-loaded nanoparticles promote the polarization …
2018年5月21日 · Here, we show that R848, an agonist of the toll-like receptors TLR7 and TLR8 identified in a morphometric-based screen, is a potent driver of the M1 phenotype in vitro and …
Frontiers | Host-functionalization of macrin nanoparticles to enable ...
2024年3月12日 · We implanted MC38-H2B-mApple tumor cells in IL12-eYFP reporter mice, which were treated with Pacific Blue-dextran as a macrophage-imaging agent to allow for the …
Expansion of tumor-associated Treg cells upon disruption of a …
2021年6月21日 · We transferred NFAT-GFP-expressing Treg cells into BMCs 3 weeks after bone marrow transfer, when the T cell compartment was not yet reconstituted, in order to enhance …
MC38结肠癌小鼠肿瘤浸润免疫细胞的动态变化 - 道客巴巴
2021年11月7日 · 方法 将小鼠结肠癌MC38细胞移植到24只C57BL/6J小鼠皮下，荷瘤后每周一次记录小鼠体质量及肿瘤大小的变化，每周一次采用流式细胞术检测肿瘤浸润免疫细胞水平，包括 …
MC-38-GFP小鼠结肠癌带绿色荧光_小鼠细胞系_细胞系_澳睿赛生 …
2024年12月2日 · 该细胞为已经稳定转染GFP的细胞，随细胞传代次数的增加，其GFP荧光强度会逐渐减弱。 若实验要求需要维持荧光强度，可以加入嘌呤霉素进行再次筛选。 1)收到细胞 …
光学显微技术和相干断层扫描技术在活体癌症研究中的应用|肿 …
2022年7月14日 · 磁共振成像（magnetic resonance imaging, MRI）和超声等临床成像技术能够对癌症的位置、解剖结构及其随时间的变化情况进行宏观测量，但无法测量病变的细胞和分子尺 …
Macrophage calcium reporter mice reveal immune cell …
We implanted 1 million MC38-H2B-mCherry colon adenocarcinoma cells into the tissue underlying the window chamber to facilitate host immune cell recruitment. Within 24–72 h of MC38-H2B …