2023年4月12日 · Here, we demonstrate that initiating HBV replication with in vitro–transcribed pgRNA can be used to study post-entry steps in the HBV life cycle with excellent signal-to-noise properties. These properties, for the first time in cell culture, enable identification of sequence variants that confer resistance to anti-HBV drugs.

2023年9月13日 · In this study, we systematically profiled HBV nucleocapsid- and pgRNA-associated cellular proteins by proteomic analysis and identified TIA-1-related protein (TIAR) as a novel cellular protein...

核cccDNA可以作为模板，合成前基因组RNA（pgRNA），以及完成乙肝病毒复制和其他亚基因组RNA的RNA中间体。 pgRNA在乙肝病毒生命周期主要有2种作用，已经被科学家发现，分别是翻译和反转录模板。 pgRNA是一种超长的转录本，包含第二个DR1拷贝、ε信号和poly-A尾巴，可以作为90 kDa病毒聚合酶 (21 kDa核心蛋白)的转录本，以及一个24 kDa的早期抗原前体。 其次，它还是乙肝病毒 (-)- dna链逆转录的模板，所以，它也是乙肝病毒复制不可或缺的组成要素。 除了 …

2022年12月21日 · Hepatitis B virus (HBV) replicates its genomic DNA by reverse transcription of an RNA intermediate, termed pregenomic RNA (pgRNA), within nucleocapsid. It had been shown that transfection of in vitro-transcribed pgRNA initiated viral replication in human hepatoma cells. We demonstrated here that viral capsids, single-stranded DNA, relaxed ...

2020年10月29日 · 该篇文章报道了细胞培养上清和患者血清中存在含有HBVpgRNA的病毒样颗粒（viron-like-particle，VLP），它们在细胞内通过pgRNA衣壳化和覆盖外包膜进行组装，并通过MVB分泌途径分泌。 病毒颗粒中的HBV RNA主要是剪接的pgRNA，并缺乏3’端序列。

Pregenomic RNA (pgRNA) is an emerging serological marker for chronic hepatitis B virus (HBV) infection. While pgRNA is principally the template for viral proteins and viral DNAs, additional novel functions for the serum pgRNA have recently been described. These results extend for pgRNA a regulatory …

2018年8月7日 · 文章讲到，该团队通过一系列研究证实血清中 hbv rna为包裹在病毒样颗粒内的未经或部分逆转录的前基因组rna（pgrna），明确了血清hbv rna的性质和来源。

2020年10月20日 · HBV is an enveloped DNA virus that replicates its DNA genome via reverse transcription of a pregenomic (pg) RNA intermediate in hepatocytes. Interestingly, HBV RNA can be detected in virus-like particles in chronic hepatitis B (CHB) patient serum and has been utilized as a biomarker for intrahepatic cccDNA activity in treated patients.

HBV pgRNA是HBV cccDNA的直接转录产物,能够通过反映HBV cccDNA的转录活动状态进而反映慢性乙型肝炎病情进展,指导临床治疗和判断预后。 与其他常用HBV感染血清标志物相比,HBV pgRNA在反映HBV复制活动和抗病毒治疗效果等方面更灵敏,对于抗病毒治疗阶段终点有预测价值。

2022年9月30日 · Human hepatitis B virus (HBV) replication is initiated by the binding of the viral polymerase (P) to epsilon (ε), an ≈85-nucleotide (nt) cis-acting regulatory stem-loop RNA located at the 5′-end of the pre-genomic RNA (pgRNA). This interaction triggers P and pgRNA packaging and protein-primed reverse transcription and is therefore an ...