2019年1月3日 · To determine whether and how exosomes from human umbilical vein endothelial cells (HUVEC-Exos) regulates vascular smooth muscle cells (VSMCs) calcification/senescence in high glucose condition. HUVEC-Exos were isolated from normal glucose (NG) and high glucose (HG) stimulated HUVECs (NG/HG-HUVEC-Exos) by super speed centrifugation.

近日，中国科学院过程工程研究所杜昱光研究员研究团队在 化学领域TOP期刊《International Journal of Biological Macromolecules》（IF=8.2/Q1） 上发表了题目为“Variations in metabolic enzymes cause differential changes of heparan sulfate and hyaluronan in high glucose treated cells on chip”的研究论文，通过微流控芯片上的血管糖萼研究HG诱导人脐静脉内皮细胞（HUVECs）中糖萼及其组成聚糖的变化。 奥维森为此提供了转录组学测序分析服务。 研究 …

2014年3月4日 · 这项研究旨在评估UCP2对人脐静脉内皮细胞（HUVEC）凋亡的作用和机制。在存在或不存在UCP2（+ / +）慢病毒转染的情况下，在正常葡萄糖（NG，5.5 mmol / l）或高葡萄糖（HG，30 mmol / l）培养基中培养HUVEC。慢病毒介导的UCP2过表达抑制了HG诱导的HUVEC的凋亡。

摘要: 目的 探讨香菇多糖 (lentinan，LNT)对高糖 (glucose，HG)诱导人脐静脉内皮细胞 (human umbilical vein endothelial cells，HUVECs)损伤的干预作用和机制。 方法 筛选HG诱导HUVEC损伤的最佳浓度后、给予不同浓度LNT治疗，检测HUVEC细胞活力、活性氧 (ROS)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)水平。 MDC染色法测定HUVEC内自噬水平。 PCR法检测自噬相关基有Beclin-1水平。 Western blot法检测各组LC3、诱导型一氧化氮合酶 (iNOS)表达和p38 MAPK磷 …

2014年5月1日 · In order to test this hypothesis, we have analyzed the transcriptome of human umbilical vein endothelial cells (HUVEC) obtained from the vein of umbilical cords collected from GD patients, and compared to control cells obtained from healthy donors. These cells are an expedient ex vivo model for the study of the molecular consequences of chronic HG.

2019年1月3日 · HG-HUVEC-Exos significantly increased LDH activity, as well as the product of lipid peroxidation (MDA content), and decreased oxidative stress marker activity, as compared with NG-HUVEC-Exos. Moreover, mechanism studies showed that mitochondrial membrane potential and the expression levels of mitochondrial function related protein HADHA and Cox ...

CircRNA-0077930 transmitted by HG-HUVEs-Exos induced senescence of VSMCs by down-regulation of miR-622 expression and up-regulation of Kras, p21, p53 and p16 expression. Moreover, the lactate dehydrogenase (LDH) activity was significantly increased while anti-oxidative stress marker (superoxide dismutase, SOD) activity was reduced in HG-HUVEC ...

2019年10月7日 · Inflammation, apoptosis, and impaired angiogenesis are implicated in the pathogenesis of high glucose (HG)-induced injury of vascular endotheliocytes. Our study is aimed at evaluating the effect of Andr on HG-induced HUVEC injury and the underlying mechanism.

高葡萄糖（hg）诱导对培养的人脐静脉内皮细胞（huvec）的氧化损伤。 最近的研究发现，衣康酸4-辛基酯（OI）是一种新型且可渗透细胞的Nrf2（核因子-E2相关因子2）激活剂。

In this study, we have investigated the mechanism (s) by which AR regulates hyperglycemia-induced endothelial dysfunction. Incubation of HUVECs with high glucose (HG) decreased the cell viability, and inhibition of AR prevented it.