2021年10月15日 · In this study, we developed functional oligonucleotides that mimic the function of 7SK (7SK mimics) as novel inhibitors of HIV replication. We defined the essential region of 7SK regarding its suppressive effects on transcriptional downregulation using an antisense strategy.

2018年10月15日 · Nature Communications - The HIV Tat protein recruits a host elongation factor from the cellular 7SK complex to the viral TAR RNA to ensure transcriptional elongation. Here, Pham et al. solve...

HIV requires P-TEFb for its replication 12, 15 and this suggests that the 7SK snRNP could be targeted for silencing and/or re-activating viral gene expression in the infected host. Other diseases that are impacted by P-TEFb include cardiac hypertrophy, steroid-dependent or responsive cancers, leukemias, lymphomas, as well as inflammatory and ...

Now D'Orso and Frankel 1 report the unexpected findings that Tat assembles with the inhibitory 7SK small nuclear ribonucleoprotein (snRNP) harboring the catalytically inactive form of P-TEFb and...

2014年5月2日 · In this study, we demonstrate that the 7SK small nuclear ribonucleoprotein (snRNP) complex is recruited to the HIV-1 promoter via newly-synthesized HIV-1 nascent transcripts (short transcripts) in an hnRNP A1-dependent manner and negatively regulates viral transcript elongation.

2016年8月24日 · Our present data support the idea that the 7SK RNP plays an inhibitory role in HIV's transcription and escape from latency. The demonstration that this RNP can be destabilized by reducing 7SK RNA pseudouridylation, which activates HIV transcription and promotes latency reversal, implicates the DKC1–box H/ACA RNPs as a promising new target for ...

The 7SK snRNA sequesters P-TEFb, a general transcription elongation factor and human co-factor for HIV-1 Tat protein, into the catalytically inactive 7SK snRNP Little is known about how 7SK RNA is regulated to perform this function. Here, we show that most of 7SK is pseudouridylated at position U250 …

The 7SK snRNP is a reservoir of nuclear P-TEFb that can be withdrawn for activated transcription in response to HIV-1 infection, stress and other growth-stimulating signals. In the nucleus, a major portion of P-TEFb is sequestered in the 7SK snRNP, where the integrity of 7SK snRNA is maintained by MePCE and LARP7 and P-TEFb’s kinase activity ...

2014年7月7日 · To analyze genetic interactions between HEXIM1 and/or LaRP7 and 7SK using a cell-based system, we established artificial heterologous RNA tethering assays in which reporter gene expression depended on interactions between selected regions of 7SK and its cognate binding partners fused to a strong activator.

2022年8月15日 · Productive transcriptional elongation of many cellular and viral mRNAs requires transcriptional factors to extract pTEFb from the 7SK snRNP by modulating the association between HEXIM and 7SK...