Pol (DNA polymerase) refers to a gene in retroviruses, or the protein produced by that gene. Products of pol include: Common to all retroviruses, this enzyme transcribes the viral RNA into double-stranded DNA. This enzyme integrates the DNA produced by reverse transcriptase into the host's genome.

2022年7月6日 · We developed a system to produce stable HIV-1 Pol and determined its cryo–electron microscopy structure. RT in Pol has a similar arrangement to the mature RT heterodimer, and its dimerization may draw together two PR …

The Pol protein of human immunodeficiency virus type 1 (HIV-1) harbours the viral enzymes critical for viral replication; protease (PR), reverse transcriptase (RT), and integrase (IN). PR, RT and IN are not functional in their monomeric forms and must come together as …

Currently available viral genotyping methods are useful for viral quantification, but are inadequate for sequence profiling or comprehensive mutation detection in the variable regions of HIV polymerase (pol). A novel set of universal primers within pol, with consensus among a variety of HIV-1 subtypes, was developed.

Our HIV-1 NL4.3 Pol dimer model is the first full-length Pol tertiary structure. It can provide a structural platform for studying the autoprocessing mechanism of HIV-1 Pol and for developing new potent drugs. Moreover, the workflow can be used to predict other large protein structures that cannot be resolved via conventional experimental methods.

2025年3月18日 · HIV-1 pol gene diversity reflected HIV-1 subtype C being the dominant subtype and the presence of potential unique recombinant forms. Evolutionary estimates indicated that these recombinants might have emerged during 2004–2005, with ancestry of their subtype B traced to be of United States of America (USA) origin. The drug resistance mutation ...

3 天之前 · Demographic characteristics of the participants. From 1,004 HIV-1 positive plasma samples, 627 gag, 809 pol and 723 env sequences were successfully amplified and sequenced. Based on the genotypes ...

4 天之前 · 本文深入探讨了HIV-1蛋白酶（PR）激活与逆转录酶（RT）稳定性及Gag-Pol二聚化之间的关系，揭示了RT在PR激活过程中的重要作用，并提出了Gag-Pol二聚化作为HIV/AIDS治疗新靶点的可能性。 引言 HIV-1是一种具有高度变异性和复杂生命周期的逆转录病毒，其复制和成熟过程依赖于多种病毒蛋白的相互作用。 Gag基因编码的多聚蛋白是病毒样颗粒（VLP）组装和释放的关键，而Pol基因编码的蛋白酶（PR）、逆转录酶（RT）和整合酶（IN）则在病毒复制中发挥 …

4 天之前 · Consequently, focusing on these proteins may represent an effective strategy for preventing or treating HIV−1 infection. Gag, Pol, and Env are examples of polyproteins that have been identified as immunogenic, meaning they have the potential to elicit a strong immune response . Moreover, HIV−1 exhibits significant genetic variability ...

Our aims were to systematically characterize HIV-1 subtype evolution and to identify the regions responsible for HIV-1 subtype differentiation at the amino acid level in the Pol protein, which is often used to classify the HIV-1 subtypes.