
微同源末端连接MMEJ简介 - 知乎
MMEJ在M期和S早期处于激活状态,HDR处于非激活状态,NHEJ、HDR和MMEJ修复的平衡因机体而异。 MMEJ并不像HDR那样产生"完美修复",但它比NHEJ更具有可预测性。
DNA损伤修复机制——非同源末端链接NHEJ和同源重组HR
2020年4月3日 · 对于DSBs损伤,主要有 非同源末端链接(Non-homologous end joining,NHEJ) 和 同源重组 (Homologous recombination,HR) 两种,单链退火修复一般发生在串联同 …
Microhomology-mediated end joining - Wikipedia
Microhomology-mediated end joining (MMEJ), also known as alternative nonhomologous end-joining (Alt-NHEJ) is one of the pathways for repairing double-strand breaks in DNA.
Homology-mediated end joining-based targeted integration using CRISPR ...
2017年5月19日 · Here we devise a homology-mediated end joining (HMEJ)-based strategy, using CRISPR/Cas9-mediated cleavage of both transgene donor vector that contains guide RNA …
Microhomology-mediated end joining: new players join the team
2017年1月13日 · MMEJ is a mutagenic DSB repair mechanism, which always associates with deletions flanking the break sites and contributes to chromosome translocations and …
中科院神经所杨辉等课题组设计出更高效精确的基因靶向整合新策略
2017年6月5日 · 该研究设计了一种以同源臂介导的末端接合(Homology-Mediated End Joining, HMEJ)为基础的基因敲入策略,它在很多种系统(体外培养的细胞、动物胚胎和体内组织) …
Nature发表基于选择性非同源末端连接修复的基因治疗方法
2019年4月4日 · NHEJ通路是大多数细胞中占主导地位的DSB修复通路。 MMEJ通路通过末端切除来发现断裂两侧的微小同源序列,这些同源序列可用于模板化断裂端融合。 PARP-1可通 …
Microhomology-mediated End Joining and Homologous …
2013年4月22日 · Using a MMEJ and HR competition repair substrate, we demonstrated that MMEJ with short end resection is used in mammalian cells at the level of 10–20% of HR when …
Microhomology-mediated end joining: a back-up survival …
Microhomology-mediated end joining (MMEJ) is an error-prone repair mechanism that involves alignment of microhomologous sequences internal to the broken ends before joining, and is …
MMEJ介导的基因编辑--大幅增加基因编辑效率 - 简书
2019年4月4日 · Gaps: The NHEJ-mediated homology-independent targeted integration (HITI) system is especially useful for in vivo gene knock-in with high efficiency. however, this …
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