The colony-forming unit (CFU) assay is one of the most widely used assays for hematopoietic stem and progenitor cells (HSPCs). CFU assays allow measurement of the proliferation and differentiation ability of individual cells within a sample.

The Colony Formation Unit (CFU) Cell Assay. The colony forming unit (CFU) cell assay, or CFC assay, is used to study the proliferation and differentiation of hematopoietic progenitors by their ability to form colonies in a semisolid medium such as methylcellulose or agar.

The StemMACS HSC-CFU Assay Kit offers a highly standardized method for analyzing hematopoietic stem and progenitor cells. The kit combines differentiation in methylcellulose-free cell culture with a standardized, flow cytometry-based read-out and eliminates the need for user-dependent, visual scoring under a microscope.

2019年4月28日 · CFU（集落形成单位）检测是对造血干细胞与祖细胞（HSPCs）分析最为广泛使用的方法之一。 CFU 检测可以量化一个样品中个别细胞的增殖以及分化能力。 这些细胞的潜能可以通过观察由每个接种的祖细胞所产生的集落（更多分化的细胞所组成）来衡量。 大约 14 天的培养足以使集落生长到允许准确计数和鉴定的大小，尽管在某些情况下培养时间可以更短。 此前，我们介绍了与 CFU 检测相关的主题，比如使用 HetaSep™ 富集的造血细胞样本的合适的铺板密 …

2021年12月17日 · Hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow and supply blood cells. Efficient methods for isolation of HSPCs are required. Here, we present protocols for the isolation of human and murine HSPCs using …

Progenitor cells have limited in vivo hematopoietic potential and must therefore be identified using in vitro assays. The CFU assay is the most widely used and best standardized in vitro assay for multipotent and lineage-restricted progenitors of most blood cell lineages.

高增殖潜能集落形成单位（High Proliferative Potential Colony-Forming Cells，HPP-CFC）和粒-红-巨噬-巨核集落形成单位（CFU-GEMM）为多潜能祖细胞，较系列特异性祖细胞CFU-GM、CFU-G、 CFU-M、CFU-Mk、BFU-E和CFU-E更为原始。

造血干细胞CFU（Colony For... 1)将所需要的MethoCult®培养基在2-8℃过夜或室温融化。 (3)准备三个35 mm的培养皿，制备双份培养系统。 具体方法如下：把两个35mm的带盖培养皿和一个盛有水的35mm无盖培养皿一起放置到100 mm的培养皿中。 (4)准备CD34 细胞，细胞计数仪计数后用0.3mlIMDM 2%FBS稀释细胞至10倍的接种终浓度。 推荐细胞数是每个3.5 cm培养皿500～2000个细胞。 然后把稀释后的细胞悬液按照1：10的比例加到MethoCult®培养基中. (5)将细胞转移到 …

StemMACS HSC-CFU media are designed to maximize growth and differentiation of progenitor cells and allow the clonal progeny of a single cell to grow in a distinct cluster or colony. They are produced under tightly controlled manufacturing conditions and use highly qualified raw materials to provide a consistent and optimally performing colony ...

StemMACS HSC Media have been developed for the expansion of CD34+ cells and to assess the differentiation potential of human hematopoietic stem and progenitor cells as Colony Forming Units (CFU). They are ideally suited for use with unseparated blood3, bone marrow4, or fetal liver cells sorted cell populations1,4 or ES and iPS cell-derived ...